A positive feedback loop between AlkB homolog 5 and miR-193a-3p promotes growth and metastasis in esophageal squamous cell carcinoma

Nov 24, 2020Human cell

A feedback loop between AlkB homolog 5 and miR-193a-3p may promote growth and spread in esophageal squamous cell cancer

AI simplified

Circadian Biology on OpenScience ↗PubMed ↗DOI ↗

Abstract

miR-193a-3p expression was significantly upregulated and associated with advanced TNM stage, recurrence, and poor prognosis in esophageal squamous cell carcinoma (ESCC) patients.

miR-193a-3p may act as an oncogene in ESCC.
ALKBH5 is targeted by miR-193a-3p, leading to its suppression.
There is a reciprocal regulatory relationship where ALKBH5 inhibits miR-193a-3p expression.
ALKBH5 negatively regulates the modification process of primary miR-193a-3p.
The feedback loop between miR-193a-3p and ALKBH5 promotes ESCC cell proliferation, migration, and invasion.
In vivo studies suggest this feedback loop facilitates tumor growth and metastasis.

AI simplified

Esophageal squamous cell carcinoma (ESCC) is one of the most frequent malignancies worldwide. miR-193a-3p acts as an oncogene or tumor suppressor in different cancers. However, the functional role and regulatory mechanism of miR-193a-3p in ESCC remain to be elucidated. Our results demonstrated that miR-193a-3p expression was significantly upregulated and associated with advanced TNM stage, recurrence, and poor prognosis in ESCC patients. miR-193-3p targeted ALKBH5 and suppressed its expression. ALKBH5 inhibited miR-193a-3p expression in turn. ALKBH5 affected the primary miR-193a-3p processing by negatively regulating its mA modification. These findings suggested a positive feedback regulation between miR-193a-3p and ALKBH5 in ESCC cells. Moreover, the functional assays indicated that the miR-193-3p-ALKBH5 feedback loop promoted the proliferation, migration and invasion ability of ESCC cells in vitro, and facilitated tumor growth and metastasis in vivo. Collectively, our current study identified a novel positive feedback regulation between miR-193a-3p and ALKBH5 in ESCC, which may be helpful to gain insight into ESCC pathogenesis and provide novel therapeutic target for ESCC. 6

Full Text

Full text is available at the source.

View full text (PDF) ↗View full text (HTML) ↗

A positive feedback loop between AlkB homolog 5 and miR-193a-3p promotes growth and metastasis in esophageal squamous cell carcinoma

Abstract

Full Text

You found one interesting study. We’ll send the next 7.

what lands in your inbox each week:

Recent issues from the circadian biology brief

Abstract

Full Text

Related papers

You found one interesting study. We’ll send the next 7.

what lands in your inbox each week:

Recent issues from the circadian biology brief