Two novel antimicrobial peptides, arasin-likeSp and GRPSp, from the mud crab Scylla paramamosain, exhibit the activity against some crustacean pathogenic bacteria

Two novel antimicrobial peptides (AMPs) were identified from the hemocytes of the mud crab, Scylla paramamosain.

• The arasin-likeSp and GRPSp peptides are encoded by open reading frames of 198 and 168 base pairs, respectively.
• Arasin-likeSp and GRPSp peptides have predicted molecular masses of 4373 Da and 2995 Da.
• Transcript levels of both peptides in healthy crabs are high in hemocytes and increase significantly within 3 hours after bacterial challenge.
• Synthetic arasin-likeSp exhibits antimicrobial activity against both Gram-positive and Gram-negative bacteria, including crustacean pathogens.
• Synthetic GRPSp shows antibacterial activity specifically against certain Gram-positive bacteria, but not against Gram-negative bacteria.

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