Novel automated sample-to-result SARS-CoV-2 laboratory-developed RT-PCR assay for high-throughput testing using LabTurbo AIO 48 system

Dec 14, 2020Clinica chimica acta; international journal of clinical chemistry

Automated rapid COVID-19 test for large-scale screening using the LabTurbo AIO 48 system

AI simplified

Quantum Physics on OpenScience ↗PubMed ↗DOI ↗

Abstract

The LabTurbo AIO 48 system produced results in approximately 2.5 hours for 48 samples, significantly faster than the 4.8 hours required by the standard RT-PCR method.

The LabTurbo AIO 48 demonstrated a limit of detection of 9.4 copies per reaction, indicating higher sensitivity compared to the reference RT-PCR assay.
An overall percentage agreement of 100% was observed between the LabTurbo AIO 48 and the standard detection method in a study of 125 clinical samples.
Immediate detection provided by the LabTurbo AIO 48 may contribute to better control and prevention measures for COVID-19.

AI simplified

BACKGROUND AND AIMS: Immediate detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is critical for preventing the spread of coronavirus disease 2019 (COVID-19). The LabTurbo AIO 48 system is an automated platform that allows nucleic acid extraction and sample analysis on the same instrument, producing faster results without affecting their accuracy. We aimed to independently evaluate the LabTurbo AIO 48 (all-in-one system) for SARS-CoV-2 detection.

MATERIALS AND METHODS: Comparative limit of detection (LOD) was assessed on both the LabTurbo AIO 48 and current standard detection system based on real-time reverse transcriptase polymerase chain reaction (RT-PCR), using SARS-CoV-2 RNA control. Additional 125 primary clinical samples were assessed using both the protocols in parallel.

RESULTS: The turnaround time from sample to results for 48 samples analyzed on LabTurbo AIO 48 was approximately 2.5 h, whereas that analyzed using the in-house RT-PCR protocol was 4.8 h. LabTurbo AIO 48 also demonstrated higher sensitivity than our reference RT-PCR assay, with a LOD of 9.4 copies/reaction. The overall percentage agreement between both the methods for 125 samples was 100%.

CONCLUSION: LabTurbo AIO 48 is a robust detection option for SARS-CoV-2, allowing faster results and, consequently, aiding in better control and prevention of COVID-19.

Full Text

We can’t show the full text here under this license. Use the link below to read it at the source.

View full text (XML) ↗View full text ↗

Novel automated sample-to-result SARS-CoV-2 laboratory-developed RT-PCR assay for high-throughput testing using LabTurbo AIO 48 system

Abstract

Full Text

You found one interesting study. We’ll send the next 7.

what lands in your inbox each week:

Recent issues from the quantum physics brief