Expressions of tyrosinase and are significantly reduced in the skin lesions of patients with advanced .
Downregulation of Pmel17 is associated with reduced melanin synthesis in melanocytes.
In a mouse model, silencing Pmel17 exacerbated the vitiligo phenotype and induced depigmentation.
Pmel17 downregulation triggers autophagy in melanocytes and inhibits the PI3K-AKT-mTOR signaling pathway.
Treatment with a PI3K/AKT inhibitor intensified the effects of Pmel17 silencing on specific protein expressions.
Upregulation of Pmel17 did not affect tyrosinase expression or melanin content in melanocytes.
Simplified
BACKGROUND: is a prevalent depigmentation skin disorder with a complex etiology and incompletely understood pathogenesis. Previous studies have suggested a potential involvement of premelanosome protein 17 () in vitiligo, although the specific mechanism remains unclear. This study aimed to explore the association between Pmel17 and vitiligo development, as well as its mechanistic actions.
METHODS: Initially, clinical samples from vitiligo patients and healthy individuals were collected to assess Pmel17 and tyrosinase (TYR) expression levels in tissues using immunohistochemistry and RT-PCR. Subsequently, the effect of Pmel17 on the vitiligo phenotype was validated in a mouse model. Finally, at the cellular level, Pmel17-siRNA was transfected into melanocytes to evaluate the effect and mechanism of Pmel17 on melanin synthesis.
RESULTS: Compared with normal skin tissues, the expressions of TYR and Pmel17 in the lesions of patients with advanced vitiligo was significantly reduced. The results of animal experiments demonstrated that Pmel17-shRNA lentivirus infection induced depigmentation in mice and exacerbated the vitiligo phenotype in monobenzone model mice. At a cellular level, down-regulation of Pmel17 expression reduced melanin synthesis and induced autophagy in melanocytes, concomitant with inhibition of the PI3K-AKT-mTOR signaling pathway. Treatment with LY294002, a PI3K/AKT inhibitor, enhanced the suppressive effects of Pmel17 down-regulation on p-AKT and p-mTOR proteins. Conversely, upregulation of Pmel17 in melanocytes did not impact TYR expression or melanin content.
CONCLUSION: The findings demonstrate that downregulation of Pmel17 contributes to the pathogenesis of vitiligo by inducing autophagy and inhibiting melanin synthesis, through the suppression of the PI3K-AKT-mTOR signaling pathway. These results provide new insights into the molecular mechanism between Pmel17 dysfunction and depigmentation.
Key numbers
n=8
Decrease in and expression
Statistical analysis of and expression in skin tissues from patients with advanced and healthy individuals.
p<0.05
Reduction in melanin synthesis
Statistical significance of melanin content following treatment in melanocytes.
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