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Abstract
A new biosensor for detecting PYY production from human enteroendocrine cells has been developed.
- Peptide YY (PYY) is associated with regulating satiety and intestinal health.
- Current human cell lines for studying PYY secretion are insufficient for mechanistic research.
- The engineered SEP-PYY biosensor responds to nutrient stimuli and utilizes native hormone processing mechanisms.
- PYY secretion can be quantified using flow cytometry and spectrofluorometric methods, which are more efficient than existing techniques.
- The biosensor enables the observation of single-event hormone release through advanced microscopy.
- The platform can be used to identify ligands that stimulate SEP-PYY secretion via specific receptors, potentially aiding in metabolic disease research.
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