Effect of carbohydrate-protein supplementation postexercise on rat muscle glycogen synthesis and phosphorylation of proteins controlling glucose storage

Glycogen concentration in the muscle was greater in rats receiving a carbohydrate plus protein supplement at 90 minutes postexercise compared to those receiving carbohydrate alone.

• Muscle glycogen levels were higher in the carbohydrate plus protein group compared to the carbohydrate-only group at 90 minutes postexercise.
• In red quadriceps, glycogen concentrations were 28.3 µmol/g for the carbohydrate plus protein group versus 22.4 µmol/g for the carbohydrate group.
• In white quadriceps, glycogen concentrations were 24.9 µmol/g for the carbohydrate plus protein group versus 17.64 µmol/g for the carbohydrate group.
• Protein kinase B phosphorylation was elevated in the carbohydrate plus protein group compared to control groups at specific time points.
• Glycogen synthase phosphorylation decreased 30 minutes postexercise and returned to baseline by 90 minutes, with no significant differences between supplement types.

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