Life science allianceJul 9, 2026

Blocking CDK4/6 causes cells to release aging-related signals by slowing activation of NF-κB

Longevity & Aging Weekly Brief ↗PubMed ↗DOI ↗OA ↗

Updated Jul 10, 2026

Abstract

Up-regulation of NF-κB-driven SASP genes was observed with both CDK4/6 inhibitors and DNA damage agents, although it was delayed with CDK4/6 inhibitors.

Cellular senescence involves a permanent exit from the cell cycle triggered by stressors such as genomic instability.
The senescence-associated secretory phenotype (SASP) can induce inflammation in surrounding cells.
CDK4/6 inhibitors can lead to a senescence-like phenotype without extensive DNA damage.
NF-κB-driven SASP gene expression was found to be associated with both CDK4/6 inhibitors and DNA damage, but with differences in timing.
Inhibiting NF-κB reduced SASP gene expression but did not reverse the stable arrest of cells.

Simplified

Cellular senescence consists of regulated phenotypes associated with permanent exit from the cell cycle in response to stressors such as genomic instability. The consequences of senescence go beyond individual cells because of the senescence-associated secretory phenotype (SASP), which can induce inflammation in neighboring cells. Some cancers respond to CDK4/6 inhibitors (CDK4/6i) with a senescence-like phenotype in the absence of extensive DNA damage. We asked how the SASP and the transcriptional regulatory profile triggered by CDK4/6i-driven arrest compare with the canonical NF-κB-regulated SASP triggered by DNA damage. We profiled the dynamics of transcriptional regulation in response to the CDK4/6i, palbociclib, and the DNA-damaging agent, doxorubicin. We found that up-regulation of NF-κB-driven SASP genes was shared across both drugs, although delayed in CDK4/6i, coinciding with slower enhancer activation and epigenetic changes. ATM/ATR inhibition did not affect CDK4/6i-induced NF-κB nuclear localization, suggesting an alternative upstream activator. Inhibiting NF-κB suppressed the expression of SASP genes without reversing stable arrest, pointing to SASP manipulation as a potential therapeutic strategy and providing insights into controversies regarding cell cycle arrest-driven SASP.

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Blocking CDK4/6 causes cells to release aging-related signals by slowing activation of NF-κB

Abstract

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