Chaperonin‐mediated winter cold response via circadian clock components in Arabidopsis

A missense mutation in CCT8 impairs vernalization and freezing tolerance in Arabidopsis.

• The TCP1 Ring Complex (TRiC) is identified as a key regulator for both vernalization and cold acclimation.
• Mutations in CCT8 lead to decreased expression of central regulators VERNALIZATION INSENSITIVE 3 (VIN3) and C-REPEAT BINDING FACTORs (CBFs).
• TRiC is essential for the accumulation of REVEILLE (RVE) transcription factors, which are important for circadian regulation.
• RVE8 specifically activates VIN3 and CBF expression through direct binding to their promoters.
• VIN3 expression is regulated by circadian rhythms and is preferentially induced by cold during the subjective day.
• These findings suggest a regulatory framework linking circadian signaling with adaptation to cold in plants.

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