circRNA_141539 can serve as an oncogenic factor in esophageal squamous cell carcinoma by sponging miR-4469 and activating CDK3 gene

Jan 28, 2021Aging

circRNA_141539 may promote esophageal squamous cell cancer by blocking miR-4469 and activating the CDK3 gene

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Circadian Biology on OpenScience ↗PubMed ↗DOI ↗

Abstract

CircRNA_141539 is significantly up-regulated in patients with esophageal squamous cell carcinoma (ESCC).

High levels of circRNA_141539 are associated with TNM stage, differentiation, and poor prognosis in ESCC patients.
Overexpression of circRNA_141539 promotes cell proliferation and invasion in ESCC cells.
Silencing circRNA_141539 inhibits both cell proliferation and invasion.
CircRNA_141539 directly binds to microRNA-4469, which negatively regulates cyclin-dependent kinase-3 (CDK3).
CircRNA_141539 may serve as a novel diagnostic and prognostic biomarker and therapeutic target for ESCC.

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The abnormal expression and regulation of circular RNA (circRNA) is involved in the occurrence and development of a variety of tumors. The current study aimed to determine the role of circRNA_141539 in esophageal squamous cell carcinoma (ESCC). CircRNA_141539 expression in ESCC was detected via circRNA chip analysis and verified via reverse transcription-quantitative PCR. Associations between circRNA_141539, patient clinicopathological characteristics and prognosis were also statistically analyzed. Additionally, the effects of circRNA_141539 on ESCC cell proliferation and invasion were assessed. A dual-luciferase assay was performed to analyze the interaction between circRNAs, microRNAs (miRs) and mRNAs. The results revealed that circRNA_141539 was significantly up-regulated in patients with ESCC. Furthermore, high circRNA_141539 expressions were significantly associated with TNM stage, differentiation and poor prognosis, revealing high diagnostic value (P<0.05). Furthermore, circRNA_141539 overexpression promoted cell proliferation and invasion, while circRNA_141539 silencing inhibited cell proliferation and invasion (P<0.05). The dual-luciferase reporter assay identified that circRNA_141539 directly binds to miR-4469 and also revealed that cyclin-dependent kinase-3 (CDK3) was negatively regulated by miR-4469. The results indicated that circRNA_141539 served as an oncogenic factor in ESCC by sponging miR-4469 and activating CDK3 expression. circRNA_141539 may present as a novel diagnostic and prognostic biomarker and a therapeutic target for patients with ESCC.

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circRNA_141539 can serve as an oncogenic factor in esophageal squamous cell carcinoma by sponging miR-4469 and activating CDK3 gene

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