Increase of collagen synthesis by obovatol through stimulation of the TGF-β signaling and inhibition of matrix metalloproteinase in UVB-irradiated human fibroblast

📖 Top 20% JournalMar 10, 2007Journal of dermatological science

Obovatol boosts collagen production by activating healing signals and blocking collagen breakdown in skin cells damaged by UVB light

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Abstract

Obovatol increased type I procollagen, TGF-beta, and Smad expression while inhibiting matrix metalloproteinase-3 (MMP-3) in UVB-irradiated human fibroblast cells in a dose-dependent manner (1-5 μM).

Obovatol is a biphenolic compound isolated from Magnolia obovata leaves.
It stimulates collagen synthesis by enhancing TGF-beta signaling.
Inhibition of MMP-3 is associated with the protective effects of obovatol against UV damage.
Obovatol also reduces the activation of AP-1 and MAP kinases following UVB exposure.
The findings suggest that obovatol may help protect skin from photo-damage.

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BACKGROUND: Alterations of the extracellular matrix (ECM) is critical in the photo and age-damaged skin. Thus any compounds keep ECM can protected from photo and aged-damaged skin. ECM is predominantly composed of type I and type III collagens in the dermis. Transforming growth factor (TGF-beta)s play important roles in cellular biosynthesis of extracellular matrix. Activator protein 1 (AP-1) and Smad are significant factors that mediate TGF-beta.

OBJECTIVE: We have investigated increasing effects of obovatol, a biphenolic compound isolated from leaves of Magnolia obovata on the collagen synthesis through stimulation of the TGF-beta signaling and inhibition of matrix metalloproteinase, thereby protect against from UV damages via maintain of collagen in the UVB irradiated human fibroblast cells.

METHODS: The fibroblasts were pretreated with obovatol for 24h and then the cells were irradiated with UVB. UVB-exposed cells were further cultured for 24h. Type I procollagen, MMP-3, TGF-beta and Smad as well as phosphorylation of MAPK family expression were determined by Western blot. The activation of AP-1 was investigated using EMSA. The released type I procollagen and TGF-beta into cell culture medium were determined by Western blot after concentration of these proteins.

RESULTS: The results showed that obovatol stimulated type I procollagen, TGF-beta, and Smad expression and inhibited matrix metalloproteinase-3 (MMP-3) in dose-dependent manner (1-5muM) in UVB-irradiated human fibroblast cells. Obovatol also inhibited UVB-induced activation of AP-1 and MAP kinases.

CONCLUSION: These results suggest that obovatol increases collagen synthesis through stimulation of the TGF-beta signaling and inhibition of matrix metalloproteinase in UVB-irradiated human fibroblast, thus obovatol could be effective against photo-damaged skin.

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