Cotton cellulose nanofiber/chitosan scaffolds for skin tissue engineering and wound healing applications

Dec 11, 2024Biomedical materials (Bristol, England)

Cotton fiber and chitosan scaffolds for skin repair and wound healing

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Regenerative Health on OpenScience ↗PubMed ↗DOI ↗

Abstract

Incorporating 1000 μg/ml of cotton cellulose nanofibers into chitosan scaffolds significantly enhanced their stability and bioactivity.

Chitosan is a polymeric biomaterial with non-antigenic and antimicrobial properties, but it often lacks sufficient mechanical strength and bioactivity.
Cotton cellulose nanofibers can improve the physicochemical properties of chitosan-based scaffolds, crucial for tissue engineering applications.
The cotton cellulose nanofiber and chitosan scaffold demonstrated improved roughness and reduced rupture strain compared to pure chitosan.
Increased concentrations of cotton cellulose nanofibers led to higher cell viability in human fibroblasts.
The scaffold effectively promoted cellular adhesion, proliferation, migration, and collagen synthesis in skin cells.

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Chitosan (CS) is a promising polymeric biomaterial for use in scaffolds forskin models and wound dressings, owing to its non-antigenic and antimicrobial properties. However, CS often exhibits insufficient physicochemical properties, mechanical strength, and bioactivity, limiting its efficacy in demanding applications. To address these challenges, cotton cellulose nanofibers (CNFs) represent a promising nanomaterial for enhancing CS-based scaffolds in tissue engineering. CNF offers superior stiffness, and mechanical properties that enhance cellular adhesion and proliferation, both crucial for effective tissue regeneration and healing. This study aimed to develop and characterize a scaffold combining cotton CNF and CS, focusing on its cytocompatibility with human fibroblasts and keratinocytes. The cotton CNF/CS scaffold was fabricated using the casting technique, and its physicochemical properties and cellular compatibility were assessed. The results demonstrated that incorporating cotton CNF significantly enhanced the stability of the CS matrix. The CS scaffold with 1000 μg mlof cotton CNF exhibited increased roughness and reduced rupture strain compared to the pure CS scaffold. The cotton CNF/CS scaffold effectively promoted the adhesion, viability, proliferation, migration, and collagen synthesis of skin cells. Notably, increased cell viability was observed in human fibroblasts cultured on scaffolds with higher concentrations of cotton CNF (100 and 1000 μg ml). Based on the findings, the cotton CNF/CS scaffold demonstrates enhanced physicochemical properties and bioactivity, making it a promising candidate for the development ofhuman skin models and wound healing dressings. in vitro in vitro in vitro -1 -1

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Cotton cellulose nanofiber/chitosan scaffolds for skin tissue engineering and wound healing applications

Abstract

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