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Abstract
A DSPE-CpG conjugate achieved 97.1% loading efficiency onto mRNA-LNPs.
- The covalent conjugation platform integrates TLR9 agonists with phospholipids to improve immune response.
- Enhanced cellular uptake was observed through clathrin-mediated endocytosis.
- The system improved lymph node targeting of the adjuvant and antigen expression in vivo.
- In the SARS-CoV-2 model, high levels of IFN-γ⁺CD8⁺ T cells and elevated specific IgG and neutralizing antibody titers were induced.
- In the HBV model, there was a significant increase in antigen-specific CD107a and FasLCTLs.
- Acute toxicity tests indicated no significant adverse effects.
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