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Fast and Efficient CRISPR/Cas9 Genome Editing In Vivo Enabled by Bioreducible Lipid and Messenger RNA Nanoparticles
Fast and Efficient Genome Editing Inside the Body Using Special Lipid and RNA Nanoparticles
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Abstract
BAMEA-O16B can achieve up to 90% knockout of green fluorescent protein expression in human embryonic kidney cells within 24 hours of mRNA delivery.
- BAMEA-O16B is a lipid nanoparticle designed to efficiently deliver Cas9 mRNA and single-guide RNA (sgRNA) into specific cells.
- The nanoparticle releases RNA in response to the reductive environment inside cells, facilitating rapid genome editing.
- In mouse models, intravenous injection of the BAMEA-O16B/Cas9 mRNA/sgRNA nanoparticle reduces proprotein convertase subtilisin/kexin type 9 levels in serum to 20% of untreated levels.
- BAMEA-O16B represents one of the most effective carriers for CRISPR/Cas9 delivery, which could enhance the potential applications of this genome editing technology.
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