Journal of the American Chemical SocietyAug 20, 2026

Newly Created Dual-Function Proteins for Targeted Protein Breakdown

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Updated Aug 21, 2026

Abstract

A 75% success rate was achieved in creating submicromolar binders targeting the antiapoptotic proteins BCL-xL and MCL-1.

Targeted protein degradation is facilitated by routing proteins to the ubiquitin-proteasome system.
Heterobifunctional molecules, such as proteolysis-targeting chimeras (PROTACs), are designed to induce protein degradation.
A stable helix-turn-helix scaffold was developed to present multiple binding sites for targeted proteins.
Computational design enhanced binding sites to increase affinity for specific cancer targets.
The designed proteins showed low micromolar affinity for KLHL20, allowing recruitment of the ubiquitin ligase machinery.
Bifunctionalized proteins successfully degraded BCL-xL in cells, promoting apoptosis.

Simplified

In targeted protein degradation (TPD), specific subcellular proteins are removed by routing them to the ubiquitin-proteasome, autophagy, or lysosome machinery. For instance, proteolysis-targeting chimeras (PROTACs) are synthetic heterobifunctional small molecules that simultaneously bind the target and an E3 ubiquitin ligase to drive ubiquitination and degradation by the proteasome. Despite considerable success, designing such molecules is challenging, and the number of currently addressable ubiquitin E3 ligases is limited. Here, we design a heterobifunctional de novo protein to trigger the degradation of a common cancer target, resulting in a desired phenotypic output. First, we developed a highly stable and adaptable helix-turn-helix scaffold for presenting multiple binding sites. Next, we use computational protein design to incorporate and embellish hot-spot-binding sites to target the antiapoptotic mediators BCL-xL and MCL-1. We show a 75% success rate for creating submicromolar binders against these targets. Crystal structures of the complexes confirmed the designed binding poses. Then, we designed short linear motifs (SLiMs) into the loop of the scaffold to recruit KLHL20 and the ubiquitin ligase machinery. These designs have low micromolar affinity for KLHL20 comparable to that of the natural SLiMs. Moreover, the bifunctionalized proteins degrade BCL-xL in cells, leading to apoptosis.

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Newly Created Dual-Function Proteins for Targeted Protein Breakdown

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