Creating a human stem cell line with controllable p16INK4a gene activation using doxycycline

Doxycycline treatment induces dose-dependent increases in p16^INK4a expression in a newly established human embryonic stem cell line.

• A doxycycline-inducible p16^INK4a knock-in human embryonic stem cell line was created by targeting a specific safe-harbor locus.
• The cell line maintained essential characteristics, including pluripotency marker expression and tri-lineage differentiation potential.
• Normal karyotype was confirmed in the generated cell line.
• This model may assist in studying p16^INK4a-associated cellular senescence across various stem cell-derived lineages.

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