OBJECTIVE: This study aimed to investigate the crosstalk between mitochondrial fission and mitophagy in cochlear stria vascularis pericytes under cisplatin-induced ototoxic conditions.
METHOD: In this study, pericytes were divided into Control group, CDDP group, and pcDNA3.1-Drp1 +CDDP group. The changes in mitochondrial ultrastructure of pericytes were observed by transmission electron microscopy; the expression of Drp1, Pink1, Parkin and LC3B proteins was detected by Western blot and immunofluorescence; the changes in co-localization of TOM20 and LC3B were detected by immunofluorescence; the changes in reactive oxygen content of pericytes were detected by DCFH-DA fluorescent probe; and the changes in mitochondrial membrane potential of pericytes were detected by JC-1 fluorescent probe.
RESULTS: The results showed that Overexpression of Drp1 in pericytes increased the expression of Drp1, Pink1, Parkin and LC3B proteins, increased the co-localization ratio of TOM20 and LC3B, decreased the content of reactive oxygen species in pericytes, increased the mitochondrial membrane potential, and improved the mitochondrial structural damage of pericytes caused by cisplatin.
CONCLUSION: Cisplatin inhibits mitochondrial division and autophagy through Drp1-Pink1-Parkin, causing damage to pericytes.
