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Dual‐Mechanism mRNA Delivery via Fluorinated‐Sorbitol Polyplexes: Enhancing Cellular Uptake and Endosomal Escape for COVID‐19 Vaccination
Improving COVID-19 mRNA Vaccines by Using Fluorinated-Sorbitol Particles to Boost Cell Entry and Release
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Abstract
The newly developed PFS formulation demonstrated successful expression of mRNA in both Raw 264.7 cells and Balb/c mice.
- PFS is a modified cationic polymer that enhances mRNA delivery through improved cellular uptake.
- The formulation utilizes sorbitol channels and caveolae-mediated endocytosis for effective delivery.
- Fluorination of the polymer aids in endosomal escape and reduces toxicity.
- Intramuscular administration of the SARS-CoV-2 spike mRNA vaccine produced immune responses comparable to Moderna's formulation.
- The vaccine effectively neutralized the Wuhan variant strain of SARS-CoV-2, as shown by PRNT testing.
- The findings indicate that PFS could be a promising candidate for developing mRNA vaccines for various diseases.
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