Evaluation of emulsion electrospun polycaprolactone/hyaluronan/epidermal growth factor nanofibrous scaffolds for wound healing

May 28, 2015Journal of biomaterials applications

Testing tiny fiber scaffolds with growth factors for healing wounds

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Regenerative Health on OpenScience ↗PubMed ↗DOI ↗

Abstract

Biodegradable nanofibrous scaffolds composed of polycaprolactone, hyaluronan, and epidermal growth factor were developed for wound healing.

The scaffolds demonstrated a core-sheath structure that was confirmed through electron microscopy.
Sustained release studies indicated that the scaffolds could effectively deliver bovine serum albumin and epidermal growth factor.
Human skin keratinocytes and fibroblasts showed increased proliferation and infiltration when cultured on scaffolds containing both hyaluronan and epidermal growth factor.
Up-regulation of wound-healing-related genes, including collagen I, collagen III, and TGF-β, was observed in scaffolds with epidermal growth factor.
In a full-thickness wound model, enhanced skin regeneration was noted with the treatment group receiving polycaprolactone/hyaluronan/epidermal growth factor scaffolds.

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Wound healing scaffolds provide cells with structural integrity and can also deliver biological agents to establish a skin tissue-specific microenvironment to regulate cell functions and to accelerate the healing process. In this study, we fabricated biodegradable nanofibrous scaffolds with an emulsion electrospinning technique. The scaffolds were composed of polycaprolactone, hyaluronan and encapsulating epidermal growth factor. The morphology and core-sheath structure of the nanofibers were characterized by scanning electron microscopy and transmission electron microscopy. The scaffolds were also characterized for chemical composition and hydrophilicity with a Fourier-transform infrared analysis, energy dispersive spectroscopy and the water contact angle. An in vitro model protein bovine serum albumin and epidermal growth factor release study was conducted to evaluate the sustained release potential of the core-sheath structured nanofibers with and without the hyaluronan component. Additionally, an in vitro cultivation of human skin keratinocytes (HaCaT) and fibroblasts on polycaprolactone/hyaluronan and polycaprolactone/hyaluronan-epidermal growth factor scaffolds showed a significant synergistic effect of hyaluronan and epidermal growth factor on cell proliferation and infiltration. Furthermore, there was an up-regulation of the wound-healing-related genes collagen I, collagen III and TGF-β in polycaprolactone/hyaluronan/epidermal growth factor scaffolds compared with control groups. In the full-thickness wound model, the enhanced regeneration of fully functional skin was facilitated by epidermal regeneration in the polycaprolactone/hyaluronan/epidermal growth factor treatment group. Our findings suggest that bioactivity and hemostasis of the hyaluronan-based nanofibrous scaffolds have the capability to encapsulate and control the release of growth factors that can serve as skin tissue engineering scaffolds for wound healing.

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Evaluation of emulsion electrospun polycaprolactone/hyaluronan/epidermal growth factor nanofibrous scaffolds for wound healing

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