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Abstract
Lysosomal inhibitors suppress cysteine-deprivation-induced ferroptosis even in autophagy-defective cells.
- Ferroptosis is linked to the breakdown of iron-dependent lipids, leading to cell death.
- Autophagic degradation of ferritin increases bioactive iron, which may promote ferroptosis.
- Clathrin-mediated endocytosis of transferrin is necessary for cysteine-deprivation-induced ferroptosis.
- Blocking endosomal acidification and the endocytic protein AP2M1 disrupts ferroptosis.
- Restoring cellular iron with ferric ammonium citrate can reinstate ferroptosis in cells lacking endocytosis.
- Direct inhibition of the enzyme glutathione peroxidase-4 does not require endocytosis or increased cellular iron to trigger ferroptosis.
Simplified
Longevity & Aging