Epigallocatechin-3-gallate inhibit replication of white spot syndrome virus in Scylla paramamosain

Jun 27, 2017Fish & shellfish immunology

Epigallocatechin-3-gallate reduces replication of white spot virus in mud crabs

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Abstract

Treatment with 1 mg/kg EGCG significantly increased the resistance of mud crabs to white spot syndrome virus (WSSV) compared with the control.

  • EGCG treatment at 1 mg/kg may enhance the survival rate of crabs challenged with WSSV.
  • A higher dose of 1.5 mg/kg EGCG killed crab hemocytes, indicating a potential toxicity at elevated levels.
  • EGCG treatment is associated with the induction of immune pathways, such as the phenoloxidase and JAK-STAT pathways.
  • EGCG + WSSV treatment significantly reduced the number of WSSV copies in crabs at 24, 48, and 72 hours post-challenge.
  • EGCG may improve innate immunity and inhibit WSSV replication by blocking the expression of late-stage WSSV genes.

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