FAM207BP, a pseudogene-derived lncRNA, facilitates proliferation, migration and invasion of lung adenocarcinoma cells and acts as an immune-related prognostic factor

Jan 12, 2021Life sciences

FAM207BP, a noncoding RNA from a pseudogene, helps lung cancer cells grow and spread and may predict immune-related outcomes

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Abstract

FAM207BP expression was distinctly higher in lung adenocarcinoma (LUAD) than in normal tissues.

High FAM207BP expression is associated with worse overall survival and disease-free survival in LUAD patients.
FAM207BP expression correlates significantly with gender differences in LUAD.
Silencing FAM207BP leads to a marked reduction in cellular viability, migration, and invasion of LUAD cells.
Higher levels of FAM207BP expression are linked to lower B cell infiltration.
Gene Set Enrichment Analysis suggests that high FAM207BP expression is related to the regulation of gene expression, while low expression may relate to immune response.

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AIMS: This study aimed to characterize the functions of pseudogene-derived long non-coding RNA (lncRNA) FAM207BP in lung adenocarcinoma (LUAD).

MATERIALS AND METHODS: Through the Cancer Genome Atlas (TCGA)-Genotype Tissue Expression (GTEx) database, FAM207BP expression was detected in LUAD and normal tissues. Overall survival (OS) and disease-free survival (DFS) analysis was presented using log-rank test or univariate Cox regression analysis. The relationships between FAM207BP expression and clinical features were analyzed. FAM207BP expression was validated in LUAD tissues and cells using RT-qPCR. Cell viability of LUAD cells was evaluated after silencing or overexpressing FAM207BP. Furthermore, migrated and invasive abilities were examined by Transwell and scratch assays. The correlation between FAM207BP expression and the immune infiltration levels was analyzed. Gene Set Enrichment Analysis (GSEA) was performed for high- and low-expression of FAM207BP using C2 collection in the Molecular Signatures Database (MSigDB) database.

KEY FINDINGS: FAM207BP expression was distinctly higher in LUAD than normal tissues. Patients with its high expression indicated worse OS and DFS time. FAM207BP expression was significantly related to gender. RT-qPCR results confirmed that FAM207BP was significantly highly expressed in LUAD tissues and cells. Knockdown of FAM207BP distinctly suppressed cellular viability, migration and invasion for LUAD cells. Also, its expression was negatively related to B cell infiltration levels. GSEA results indicated that high FAM207BP expression was involved in regulation of gene expression. Its low expression was related to immune response.

SIGNIFICANCE: Pseudogene-derived lncRNA FAM207BP could induce proliferation and migration of LUAD cells, which could act as an immune-related prognostic factor.

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FAM207BP, a pseudogene-derived lncRNA, facilitates proliferation, migration and invasion of lung adenocarcinoma cells and acts as an immune-related prognostic factor

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