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Abstract
Recent advances in nucleic acid delivery technologies have enabled new strategies for RNA labelling.
- The main challenge for RNA therapeutics is delivering RNA effectively to the cytosol.
- Current literature focuses on improving the escape of internalized RNA from endosomes.
- Fluorescence labelling of RNA enables experimental measurement of RNA location and protein production.
- Recent developments in fluorescent nucleic acid chemistry have introduced diverse labelling strategies.
- A selection guide is provided for researchers designing assays to measure RNA delivery.
- The review discusses recent uses of fluorescently labelled RNA in delivery studies and explores future applications.
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