Fluorescent protein ticker tape: Recording multiple gene activity patterns in living cells and animals

Fluorescent protein-based ticker tapes (FPTT) logged dose-dependent, reversible endogenous cFos transcriptional histories in hippocampal neurons at 3-hour resolution over 8 days.

• FPTT allows for multiplexed recording of physiological activities in living cells.
• The technology integrates multispectral fluorescent proteins with self-assembling protein fibers.
• Quantification of cFos and NF-κB cross-talk in neurons was achieved.
• STAT3/cAMP dynamics were tracked during mouse liver injury.
• Unexpected NFAT/STAT3 cross-talk was discovered.
• Cell cycle-dependent oscillating mTOR dynamics were characterized, and four major pathways were analyzed during T cell activation.

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