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Abstract
Fluorinated modification of lipid nanoparticles can enhance mRNA expression efficiency nearly 2-fold in primary dendritic cells.
- Fluorinated lipid modification accounts for only 1.5% of the lipids in the nanoparticles.
- This modification can mediate a 5-fold enhancement of mRNA expression efficiency in tumor cells.
- Mechanistic studies indicate that fluorinated lipids promote cellular uptake of nanoparticles and help them escape endosomes.
- Overall mRNA expression is augmented at least 3-fold with fluorinated nanoparticles compared to nonfluorinated counterparts at low doses.
- Fluorinated lipid nanoparticles demonstrate favorable biosafety profiles in both cellular and animal models.
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