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Abstract
ATP3 is a novel chemical probe that provides high-contrast imaging of autophagic flux without genetic manipulation.
- ATP3 engages directly with autophagic vesicles, allowing for reliable visualization of autophagic processes.
- This probe shows minimal background fluorescence and significant signal amplification when engaged by autophagy.
- ATP3 demonstrates superior performance compared to the conventional mRFP-GFP-LC3 assay in both imaging contrast and staining uniformity.
- Dynamic monitoring with ATP3 in an oxygen-glucose deprivation model indicates a progressive enhancement of autophagic flux under ischemic conditions.
- ATP3 is positioned as a versatile tool for studying autophagy in various physiological and pathological contexts.
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