Fracture healing potential of tetracalcium phosphate: An experimental study in a rat femur standardized diaphyseal osteotomy with open approach.

Dec 14, 2025Joint diseases and related surgery

Tetracalcium phosphate's ability to help bone heal after a controlled thigh bone break in rats

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Regenerative Health on OpenScience ↗PubMed ↗DOI ↗

Abstract

Radiological scoring indicated significantly higher fracture healing in the TTCP group compared to the fracture control group on Days 14 and 28 (p<0.05).

TTCP application resulted in reduced inflammation and fibrosis at the fracture site.
Increased chondrocyte activity and neovascularization were observed in the TTCP group.
Serum levels of tumor necrosis factor-alpha (TNF-α) were significantly higher in the fracture control group compared to the TTCP and normal control groups.
TTCP did not cause any adverse tissue reactions in the treated rats.

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OBJECTIVES: This study aims to evaluate the impact of tetracalcium phosphate (TTCP) applied directly to the fracture line in a rat femur fracture model.

MATERIALS AND METHODS: Thirty-three male Sprague-Dawley rats weighing 350 to 400 g were randomly and equally divided into three groups: normal control (NC), fracture control (PC), and TTCP treatment group. Standardized femur fractures were created in the PC and TTCP groups and fixed using intramedullary Kirschner wires. In the TTCP group, TTCP dissolved in distilled water was applied directly to the fracture line. All rats were sacrificed on postoperative Day 28. Radiological, histopathological, and biochemical analyses were performed to assess fracture healing.

RESULTS: Radiological scoring revealed significantly higher fracture healing in the TTCP group compared to the PC group on Days 14 and 28 (p<0.05). Histopathological analyses showed reduced inflammation and fibrosis, and increased chondrocyte activity and neovascularization in the TTCP group. On Day 28, serum tumor necrosis factor-alpha (TNF-α) levels were significantly higher in the PC group compared to TTCP and NC, while TTCP values were comparable to NC. No adverse tissue reactions were observed in any group.

CONCLUSION: The TTCP enhances fracture healing when applied directly to the fracture line, without causing soft tissue irritation or histologically detectable adverse reactions. Its biological activity and ease of application suggest that TTCP may be a promising adjunct in the treatment of complex fractures.

Fracture healing potential of tetracalcium phosphate: An experimental study in a rat femur standardized diaphyseal osteotomy with open approach.

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