Scientific reportsNov 6, 2025

FZD6 helps miR-199a-5p promote fat cell formation in bone marrow stem cells from aplastic anemia

Regenerative Health Weekly Brief ↗PubMed ↗DOI ↗OA ↗

Updated Jan 6, 2026

Abstract

Decreased expression of FZD6 is associated with enhanced adipogenic differentiation of in .

miR-199a-5p plays a significant role in adipogenic differentiation of bone marrow mesenchymal stem cells (BM-MSCs) derived from aplastic anemia.
FZD6 and CELSR1 have been identified as promising targets of miR-199a-5p in this process.
FZD6 expression is significantly lower in adipogenic differentiation and samples from aplastic anemia patients.
A negative correlation exists between FZD6 expression and miR-199a-5p levels.
FZD6 acts as a negative regulator of adipogenesis, where its overexpression reduces the effect of miR-199a-5p on adipogenic differentiation.

Simplified

Accumulating evidence indicates that enhanced adipogenic differentiation of (BM-MSCs) derived from (AA) could contribute to the excessive fat accumulation in the bone marrow. However, the underlying molecular mechanism remains to be determined. Here in this study, we further characterized the contributing role of miR-199a-5p in adipogenesis of AA BM-MSCs through specific inhibition of miR-199a-5p expression. Subsequently, we performed a systematic screening of the downstream targets of miR-199a-5p through web tool prediction, profiling data analysis, and experimental validation, with FZD6 and CELSR1 identified as the most promising targets of miR-199a-5p in the adipogenesis of AA BM-MSCs. Finally, we mainly focused on FZD6, which presents prominently decreased expression in adipogenic differentiation and AA BM-MSC samples and negatively correlates with miR-199a-5p expression. Besides, FZD6 is demonstrated as a negative regulator of adipogenesis of BM-MSCs, and overexpression of FZD6 alleviates the facilitating role of miR-199a-5p in the adipogenic differentiation of BM-MSCs. Overall, our findings provide further mechanistic insights into miR-199a-5p-mediated enhanced adipogenesis of AA BM-MSCs, implicating miR-199a-5p and FZD6 as potential intervention targets for improving BM-MSC-mediated microenvironments.

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Competing interests

Declarations. Competing interests: The authors declare no competing interests.

PubMed

FZD6 helps miR-199a-5p promote fat cell formation in bone marrow stem cells from aplastic anemia

Abstract

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Funding

Competing interests

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