What this is
- This research assesses the prevalence of () symptoms among university students in the Amhara region of Ethiopia.
- It identifies associated factors influencing these symptoms, such as age, sex, residence, and lifestyle choices.
- The study involved 846 students and utilized a self-administered questionnaire to gather data.
Essence
- Approximately 32.1% of university students in the Amhara region experience symptoms. Key associated factors include age, gender, urban residence, and the use of antipain medications and soft drinks.
Key takeaways
- The prevalence of symptoms among university students is 32.1%. This indicates a significant health concern within this population.
- Students aged 20–25 years have 1.74× higher odds of experiencing symptoms compared to those under 20 years. This suggests that age-related factors may contribute to symptom severity.
- Females are 1.67× more likely to report symptoms than males. This gender disparity may be influenced by lifestyle and hormonal factors.
- Urban dwellers have a 33% reduced chance of experiencing symptoms compared to rural students. This may be due to better access to healthcare and healthier lifestyle choices.
- Students using antipain medications are 2.47× more likely to report symptoms. This highlights the need for awareness about medication side effects.
- Soft drink consumption is associated with a 1.58× increase in the likelihood of symptoms, indicating dietary habits play a crucial role.
Caveats
- The cross-sectional design limits the ability to establish cause-and-effect relationships between risk factors and symptoms.
- The study's reliance on self-reported data may introduce bias, as participants might underreport or misinterpret their symptoms.
- The use of a single diagnostic tool (GERDQ) may lead to false positives or negatives, affecting the accuracy of prevalence estimates.
Definitions
- Gastroesophageal reflux disease (GERD): A chronic condition where gastric acid flows back into the esophagus, causing symptoms like heartburn and regurgitation.
AI simplified
Introduction
Gastroesophageal reflux disease (GERD) is a chronic medical condition resulting from the reflux of gastric acid contents into the esophagus and throat or mouth to cause distressing symptoms /complications [1, 2]. It also develops due to sensorimotor disorder associated with impairment of the normal anti-reflux mechanisms and with changes in normal physiology [3].
The pooled global prevalence of GERD is 14% [4]. This magnitude varies by region, ranging from 2.5 to 33.1%, in North America, Europe, East Asia, the Middle East, Australia, and South America population [5]. The prevalence of GERD ranges from 11.8 to 52.6% among university students [6–13].
Gastroesophageal reflux disease is a potentially serious condition with risks of complications like stricture of the esophagus, Barrett’s esophagus (pre-cancerous lesion), and malignancy, [14] and could be turned into a life-threatening disease [15]. It had extra-esophageal complications such as chronic cough, chronic laryngitis, asthma, and dental erosions [2]. Due to its chronic pain, and persistent and disruptive symptoms, GERD can impair physical and mental health-related quality of life, workplace productivity (daily tasks), social function, sleep, and diet, as well as cause anxiety and depression [7, 10, 16, 17]. Gastroesophageal reflux disease can cause an economic burden due to the disease’s diagnostic and therapeutic management [11]. Heartburn, regurgitation of food, vomiting, and regurgitation during sleep [18] are the most common symptoms of GERD. Based on these symptoms, a clinical diagnosis of GERD could be made [19].
Sociodemographic, lifestyle, dietary, and behavioral, as well as psychological factors, are associated with GERD [4, 19–23]. These were sex, age, residence, [7, 11, 18, 19, 24–32] sleeping within 1 h of dinner [8, 20, 24].
Consumption of caffeinated and soft drinks, [8, 9, 18, 24, 33–43] and types of food consumption [8, 35, 37–39, 41–44]. Inadequate sleep, [7, 20] smoking, [8, 11, 19, 23–25, 28, 31, 36, 40, 41, 44–47] history of use of non-steroidal anti-inflammatory drugs or analgesics, [20, 21, 28, 32, 35, 45, 47–50], and alcohol consumption, [19, 20, 23, 39, 40, 46, 51] were associated with GERD symptoms.
Even though GERD symptoms can negatively impact one’s quality of life, daily tasks, and the country’s economy by requiring the purchase of medication to alleviate GERD symptoms in university students, the burden of GERD symptoms in Ethiopian university students has not been quantified. As a result, this research aimed to assess the prevalence of GERD symptoms and its associated factors among university students in Ethiopia’s Amhara area. Stakeholders will use the outcomes of this study to develop illness prevention, care, and early treatment methods.
Methods
Study settings, period, and design
An institutional-based cross-sectional study was employed in Amhara national regional state Universities, from April 1, 2021, to May 1, 2021. Amhara’s national regional state is in North Ethiopia and its capital city is Bahir Dar. There are 10 government-owned Universities in Amhara national regional state and, from these Universities; three of them were selected by using the lottery method for the study; namely, the University of Gondar, Wollo, and Woldia University.
Source and study population
All government university students in the Amhara region were the source population, and all regular undergraduate students registered in the University of Gondar, Wollo, and Woldia University in the 2020/21 academic year were the study population.
Sample size determination and sampling procedure
The sample size was calculated using single population proportion formula, with the assumption of 95% CI (Za/2 = 1.96), 5% (α = 0.05) level of significance, 5% (d = 0.05) margin of error, and proportion of 50% (no study done about GERD in Ethiopia), accordingly, the calculated sample size for the study became 384.
After adding a 10% non-response rate, the calculated sample size was 423. Since we have used multistage random sampling, this sample was multiplied by the design effect of two. Therefore, the final total sample size for this study was 846.
Three Universities were chosen using a simple random selection methodology (lottery method) out of the total Universities in the Amhara region. There were 25,272 undergraduate regular program students registered at the three selected Universities. The University of Gondar had 9,607 students, whereas Wollo University had 8,866 students and Woldia University had 6,799 students. Three hundred and twenty-two students from the University of Gondar, 297 from Wollo University, and 227 from Woldia University were chosen using proportionate allocation. After that, a proportional sample of these universities was assigned to the departments (health and non-health). A simple random sample procedure was used to pick research participants from each department (Fig. 1).
Schematic representation of sampling procedure for the prevalence and associated factors of GERD among university students in Amhara region, Ethiopia, 2021
Study variables
The dependent variable was gastroesophageal reflux disease. Students were said to have GERD symptoms when the Gastroesophageal reflux disease questionnaire score was ≥ 8 [52].
The independent variables were age, sex, marital status, year of study, department, residence, types of food consumed, eating habits, skipping breakfast, the timing of sleep soft drink consumption, sleep pattern (length of sleep per day), alcohol consumption, cigarette smoking, khat chewing and history of use of non-steroidal anti-inflammatory drugs or analgesics.
Operational definitions
Data collection tools and procedures
A semi-structured self-administered questionnaire and the Gastroesophageal reflux diseases questionnaire (GERDQ) were used to collect data. The GERDQ was a patient-centered, self-assessment tool that helped doctors diagnose, manage, and assess GERD symptoms without requiring an initial expert referral or endoscopy [52]. It also had diagnostic value in an unselected population presenting with typical and/or atypical GERD symptoms [53]. The participants were asked to recall their symptoms and the frequency with which they occurred throughout the previous seven days, according to GERDQ. Positive symptoms such as heartburn, regurgitation (reflux), heartburn and reflux disrupting sleep at night, and the need for further drugs were rated as follows: 0, 1, 2, and 3 points for 0 days, 1 day, 2–3 days, and 4–7 days, respectively. Second, the frequency of negative symptoms (upper abdominal pain and nausea): 3, 2, 1, and 0 points for 0 days, 1 day, 2–3 days, and 4–7 days, respectively.
The sum of the points for these frequencies served as a subject’s GERDQ scores, and GERD symptoms were diagnosed if the sum was greater than or equal to 8 points [52]. Six BSc Nurses and three BSc Public Health professionals were involved in data collection and supervision, respectively. Before collecting data, select study participants from health and non-health department based on the proportional allocation method from these selected universities. Then, select the study participants by lottery method based on their identification number which is obtained from each university’s registrar. Then after, the data collectors inform the study participants about the study’s purpose and objectives. Provide instruction to the students so they can fill out the questionnaires correctly, comprehend the questions, and avoid writing their names or other unneeded information about themselves.
Data quality control
The questionnaire was translated from the English version into the local language (Amharic version) and then retranslated back to English to ensure consistency. A pretest was conducted on 5% of the sample at Mekdela Amba University students. Appropriate training was given to the data collectors and supervisors. The collected data were checked for completeness, consistency, and accuracy on daily basis by the principal investigator.
Data processing and analysis
Data were entered into Epi Data version 4.6.0.5 and then, exported into SPSS version-26 software for data analysis. Bivariable binary logistic regression analysis was performed to determine the associated factors of GERD symptoms.
All variables with a p-value ≤ 0.25 at bivariable binary logistic regression analysis were entered into the multivariable binary logistic regression model. The strength of association was described by computing the odds ratio with a 95% confidence interval (CI). Variables having a p-value ≤ 0.05 in the final model were considered statistically significant.
Results
Sociodemographic characteristics of the respondents
Eight hundred and forty-six students were included in the study and the overall response rate was 93.5%. The age of the respondents ranges from 19 to 30 years with a median of 22 years and an interquartile range of two (2). Most of the respondents 464 (58.7%) were males and 486 (61.5%) were Orthodox Christianity followers. Most of the respondents, 705 (89.2%)) were unmarried and 648 (81.9%) were from the non-health department. Nearly half of the respondents (53.4%) were urban dwellers and 290 (36.7%) were a second year (Table 1).
| Variables | category | Frequency | Percent (%) |
|---|---|---|---|
| Age(years) | < 20 | 133 | 16.8 |
| 20–25 | 619 | 78.3 | |
| > 25 | 39 | 4.9 | |
| Sex | Female | 327 | 41.3 |
| Male | 464 | 58.7 | |
| Religion | Orthodox | 486 | 61.4 |
| Muslim | 146 | 18.5 | |
| Catholic | 38 | 4.8 | |
| Protestant | 114 | 14.4 | |
| Adventist | 7 | 0.9 | |
| Residence | Rural | 369 | 46.6 |
| Urban | 422 | 53.4 | |
| Marital status | Married | 85 | 10.7 |
| Unmarried | 706 | 89.3 | |
| Year of Study | 2 | 290 | 36.6 |
| 3 | 283 | 35.8 | |
| 4 | 178 | 22.5 | |
| 5 | 40 | 5.1 | |
| Department | Health | 143 | 18.1 |
| Non-health | 648 | 81.9 |
Lifestyle, dietary, and behavioral characteristics of the respondents
Of the total respondents, 542 (68.6%) took fasting food frequently and almost three-fourths (76.1%) of the respondents take food from university cafes. Nearly half of the respondents (52%) had the habit of eating quickly and 51.7% did not skip breakfast. Three-fourths of the respondents (74.5%) had a sleep after dinner for more than or equal to 2 h. Regarding tea and coffee consumption, 403 (51%) and 481(61%) of the respondents did not frequently drink tea and coffee, respectively. Most of the respondents (63.5%) did not take soft drinks frequently. Most of the respondents were not smokers (93.4%) and khat chewers (90.1%). Most of the respondents (62.4%) were not alcohol drinkers. Half of the respondents (50.9%) had a sleep duration of fewer than seven hours, and 646 (81.7%) students did not use antipain medication (Table 2).
| Variables | Category | Frequency | Percent (%) |
|---|---|---|---|
| Types of food consumed | Fasting | 542 | 68.5 |
| Spicy | 96 | 12.1 | |
| Fatty | 73 | 9.2 | |
| Fried and cooked | 80 | 10.1 | |
| Place of feeding | University cafe | 602 | 76.1 |
| Non-cafe | 189 | 23.9 | |
| Skip of breakfast | Yes | 382 | 48.3 |
| No | 409 | 51.7 | |
| Time of sleep after dinner | < 2 h | 202 | 25.5 |
| 2 h≥ | 589 | 74.5 | |
| Quick eating | Yes | 411 | 52 |
| No | 380 | 48 | |
| Smoke cigarette | Yes | 53 | 6.6 |
| No | 738 | 93.4 | |
| Alcohol consumption | Yes | 298 | 37.6 |
| No | 493 | 62.4 | |
| Length of sleep per day | < 7 h | 403 | 50.9 |
| 7 h≥ | 388 | 49.1 | |
| Use antipain | Yes | 145 | 18.3 |
| No | 646 | 81.7 | |
| Types of antipain used | Paracetamol | 93 | 64.1 |
| NSAIDs/analgesics (diclofenac, ibuprofen) | 52 | 35.9 | |
| Chew khat | Yes | 78 | 9.9 |
| No | 713 | 90.1 | |
| Consumption of tea | Yes | 387 | 48.9 |
| No | 404 | 51.1 | |
| Consumption of coffee | Yes | 309 | 39.1 |
| No | 482 | 60.9 | |
| Soft drink consumption | Yes | 289 | 36.5 |
| No | 502 | 63.5 |
Prevalence of gastroesophageal reflux diseases symptoms
The prevalence of GERD symptoms among university students in the Amhara region was 32.1%(95%CI = 28.7–35.5%). Regarding positive symptoms of GERD, 45.5% of the respondents had heartburn, 40.4% had regurgitation, 39% had sleep disturbance due to heartburn and regurgitation, and 18.2% used medication for relief from heartburn and regurgitation in the previous week. Concerning negative symptoms of GERD symptoms, 54.8% and 61% of the respondents had not experienced epigastric pain and nausea in the previous week, respectively (Table 3).
| Symptoms | Category | Frequency | Percent (%) |
|---|---|---|---|
| Heartburn | None /week | 431 | 54.5 |
| Once/week | 120 | 15.1 | |
| 2–3 days/week | 177 | 22.4 | |
| 4–7 days/week | 63 | 8 | |
| Regurgitation | Zero-day/week | 472 | 59.6 |
| One day/week | 169 | 21.4 | |
| 2–3 days/week | 117 | 14.8 | |
| 4–7 days/week | 33 | 4.2 | |
| Epigastric pain | None /week | 434 | 54.8 |
| One day/week | 157 | 19.9 | |
| 2–3 days/week | 142 | 18 | |
| 4–7 days/week | 58 | 7.3 | |
| Nausea | None /week | 483 | 61 |
| One day/week | 184 | 23.3 | |
| 2–3 days/week | 101 | 12.8 | |
| 4–7 days/week | 23 | 2.9 | |
| Sleep disturbance | Zero-day/week | 484 | 61.1 |
| None /week | 179 | 22.7 | |
| 2–3 days/week | 107 | 13.5 | |
| 4–7 days/week | 21 | 2.7 | |
| Use medication for relief from heartburn and regurgitation | None /week | 647 | 81.8 |
| One day/week | 96 | 12.2 | |
| 2–3 days/week | 36 | 4.6 | |
| 4–7 days/week | 12 | 1.5 |
Factors associated with gastroesophageal reflux disease
On bivariable binary logistic regression analysis, age, sex, residence, year of study, types of food consumed, place of feeding, skip breakfast, cigarette smoking, chewing khat, use of anti-pain, frequent use of coffee and tea, and soft drink were associated with GERD symptoms (p ≤ 0.25).
In multivariable binary logistic regression analysis, age, place of residence, sex, use of antipain, and soft drink were significantly associated with GERD symptoms (p ≤ 0.05). The odds of having GERD symptoms were 1.74 times higher among the age group of 20–25 years compared to those respondents aged less than 20 years (AOR = 1.74, 95% CI = 1.03–2.94). Females had a 1.67 times higher chance of having GERD symptoms than males (AOR = 1.67, 95% CI = 1.15–2.41). Students who came from urban areas had a 33% reduced chance of having GERD symptoms than rural dwellers (AOR = 0.67, 95% CI = 0.48–0.94). Students who had a history of antipain use were 2.47 times more likely to have GERD symptoms than their counterparts (AOR = 2.47, 95% CI = 1.65–3.69). Students who use soft drinks were 1.58 times more likely to have GERD symptoms than those who did not use a soft drink (AOR = 1.58, 95% CI = 1.13–2.20) (Table 4).
| Variables | Category | GERD symptoms status | Odds Ratio (95% of CI) | ||
|---|---|---|---|---|---|
| Yes (%) | No (%) | COR | AOR | ||
| Age(years) | < 20 | 35 (26.3) | 98 (73.7) | 1 | 1 |
| 20–25 | 208(33.6) | 411 (66.4) | 1.42(0.93–2.16) | 1.74(1.03, 2.94) * | |
| > 25 | 11 (28.2) | 28 (71.8) | 1.1 (0.50–2.44) | 1.32(0.53–3.31) | |
| Sex | Female | 128(39.1) | 199 (60.9) | 1.73(1.28–2.33) | 1.67(1.15,2.41) *** |
| Male | 126(27.2) | 338 (72.8) | 1 | 1 | |
| Residence | Rural | 126(34.1) | 243 (65.9) | 1 | 1 |
| Urban | 128(30.3) | 294 (69.7) | 0.84 (0.62–1.13) | 0.67(0.48,0.94) * | |
| Type of food consumed | Fasting food | 169(31.1) | 373 (68.9) | 1 | 1 |
| Spicy food | 33 (34.4) | 63(65.6) | 1.12 (0.67–1.88) | 0.87(0.52,1.45) | |
| Fatty food | 29 (39.7) | 44(60.3) | 1.30 (0.68–2.47) | 1.13(0.64,1.99) | |
| Fried and cooked | 23 (28.7) | 57(71.3) | 1.63 (0.83–3.21) | 0.69(0.39,1.24) | |
| Place of food take | University cafe | 184(30.6) | 418(69.4) | 0.75 (0.53–1.05) | 1.08(0.71,1.63) |
| Non-cafe | 70 (37.0) | 119 (63.0) | 1 | 1 | |
| Skip breakfast frequently | Yes | 132(34.6) | 250 (65.4) | 1.24 (0.92–1.68) | 1.05(0.75,1.46) |
| No | 122(29.8) | 287 (70.2) | 1 | 1 | |
| Smoke cigarette | Yes | 23 (43.4) | 30 (56.6) | 1.68 (0.96–2.96) | 1.58(0.78,3.22) |
| No | 231(31.3) | 507 (68.7) | 1 | 1 | |
| Use pain killer | Yes | 79 (54.5) | 66 (45.5) | 3.22 (2.23–4.66) | 2.47(1.65,3.69) *** |
| No | 175(27.1) | 471 (72.9) | 1 | 1 | |
| Chew Chat | Yes | 32 (41.0) | 46 (59.0) | 1.54 (0.95–2.48) | 1.25(0.69,2.28) |
| No | 222(31.1) | 491 (68.9) | 1 | 1 | |
| consumption of tea | Yes | 141(36.4) | 246 (63.6) | 1.48 (1.09–1.99) | 1.18(0.85,1.64) |
| No | 113(28.0) | 291(72) | 1 | 1 | |
| consumption of coffee | Yes | 117(37.9) | 192 (62.1) | 1.54(1.13–2.08) | 1.23(0.88,1.72) |
| No | 137(28.4) | 345 (71.6) | 1 | 1 | |
| Consumption of soft drink | Yes | 119(41.2) | 170 (58.8) | 1.90(1.40–2.59) | 1.58(1.13,2.20) ** |
| No | 135(26.9) | 367(73.1) | 1 | 1 | |
| Year of Study | 2 | 97 (33.4) | 193 (65.6) | 1 | 1 |
| 3 | 90 (31.8) | 193 (68.2) | 2.37(1.01–5.55) | 1.04(0.71,1.53) | |
| 4 | 60(33.7) | 118(66.3) | 2.20 (0.94–5.16) | 1.06(0.69,1.62) | |
| 5 | 7 (17.5) | 33 (82.5) | 2.40(1.00-5.74) | 0.48(0.20,1.20) | |
Discussion
This study aimed to determine the prevalence of Gastroesophageal reflux disease symptoms, and its associated factors among university students in the Amhara national regional state, Ethiopia. Accordingly, the prevalence of Gastroesophageal reflux disease symptoms in this study was 32.1% (95% CI = 28.7–35.5%). This study finding is consistent with studies from Nigeria (32.8%) [12], Saudi Arabia (33.18%) [25], and India (30%) [9]. This study’s findings were lower than those of previous studies in Sri Lanka (52%) [7], and Saudi Arabia ( 52.6%) [8]. It is, however, higher than studies from Iran (19.3%), [34] and Saudi Arabia (25.9%) [42], Syria (16%) [36], India (14.4%) [10], Italy (26.2%) [11], Brazil (11.8%) [6], and Nigeria (26.34%) [13]. Lifestyle, socioeconomic, and demographic differences in the study population may have contributed to these disparities [4, 54–56].
Regarding associated factors, age, sex, residence, use of antipain, and consumption of soft drinks were significantly associated with GERD symptoms among university students. According to the finding of this study, the participants aged 20–25 years were more likely to have GERD symptoms than those under the age of 20 years old. This finding was consistent with prior findings in the literature [19, 26, 31]. Justification might be that students are under academic pressure, and lifestyle changes may have an impact on the student’s physical and mental health by causing psychological stress and it causes raising gastric acid secretion, decreasing gastric emptying, and enhancing the gastric mucosa’s sensitivity to acid, psychological stress can exacerbate the symptoms of GERD [17].
And also, students are more likely to participate in risky habits like chewing chat, cigarettes smoking, drinking alcohol, sleeping too little, and drinking soft drinks and coffee to relieve stress. Due to decreased lower esophageal sphincter tone, increased acid secretion, irritation of the esophageal mucosa, decreased gastroesophageal motility, and decreased production of bicarbonate-rich saliva, these activities may exacerbate the symptoms of GERD [57–60].
Another factor significantly associated with GERD symptoms is the sex of the respondents. Females had an increased chance of having GERD symptoms compared to males. This finding is consistent with other studies conducted elsewhere [7, 11, 24, 26–30]. The possible reason might be gender-related variation, which influences eating habits and lifestyle factors, as well as hormonal effects (such as progesterone) [54, 61]. Another possible explanation might be females are more prone to stressful conditions than males. Stress activates the hypothalamic-pituitary-adrenal axis to produce cortisol and decreases the production of prostaglandins, increasing stomach acid production, and slowing gastric emptying [62].
Findings from this study revealed that respondents who came from urban were less likely to have GERD symptoms than rural dwellers. One possible reason is that urban dwellers are more aware of good living styles, dietary habits, and easier access to healthcare systems and health-related information than rural dwellers, all of which may reduce the risk of GERD symptoms.
The respondents who used analgesics were more likely to have GERD symptoms compared to those who did not. The finding of this study was consistent with many studies conducted elsewhere [20, 21, 28, 32, 35, 45, 47–50]. The possible reason might be analgesics, directly and indirectly, disrupts physiologic mucosal protection mechanisms in the digestive tract by inhibiting cyclooxygenase enzymes. Besides, analgesics might reduce lower esophageal sphincter pressure, delay emptying of the stomach, and increase acid secretion [4, 50, 63, 64].
Respondents who use soft drinks were more likely to have GERD symptoms than their counterparts. The finding of this study was in line with other findings [8, 9, 18, 24, 25, 38, 39, 41–43]. The possible reason might be soft drinks contain caffeine, gaseous, carbohydrates (sweeteners), and acid [57], which can affect the upper digestive system by causing gastric distention due to the carbonation process, which increases acid reflux by decreasing lower esophageal sphincter pressure, increasing the frequency of transient lower esophageal sphincter relaxation then push stomach acid contents back to the esophagus, increases postprandial acid exposure of the esophagus. Moreover, it might be stimulated by extra gastric acid secretion and acid pocket formation increases gastric acid contents because soft drinks have taken during a meal and after the meal, which results in GERD symptoms [57, 65–67].
This study has limitations. Because the study was cross-sectional, it is difficult to demonstrate cause-and-effect relationships between independent and dependent variables. Because of the significant overlap between dyspepsia and GERD symptoms, dyspepsia cannot be ruled out. Furthermore, because only GERDQ was used for diagnosis, there are a significant number of false positives and false negatives.
Conclusion
Approximately one-third of university students suffer from gastroesophageal reflux disease symptoms. Age, gender, residence, antipain use, and soft drink consumption were all significantly associated factors. To reduce disease burden, it is recommended to reduce modifiable risk factors such as antipain medication use and soft drink consumption.
Acknowledgements
We would like to thank the study participants, data collectors, supervisors, and colleagues.
Abbreviations
Author contributions
Mekonen Belete participated in conceptualization, data curation, formal analysis, investigation, methodology, project administration, visualization, writing the original draft, editing, and reviewing the paper.Winta Tesfaye contributed to conceptualization, data curation, investigation, project administration, software, supervision, visualizing and writing the original draft, and editing and reviewing the draft. Adugnaw Adane participated in data curation, formal analysis, investigation, methodology, software, review & editing of the paper.Yonas Akalu contributed to the following works investigation, software, supervision, visualization review, and editing of the paper.Yigizie Yeshaw partaken in conceptualization, data curation, formal analysis, investigation, methodology, software, supervision, writing – original draft, and review & editing of the paper.
Funding
For this research paper, we did not receive any grant from any funding agency.
Availability of materials
The datasets used and/or analyzed during the current study are available from the corresponding author upon reasonable request.
Declarations
Ethical approval and consent to participate
The study was conducted following Helsinki Declaration. The Ethical clearance was obtained from the institutional review board of the School of Medicine, College of Medicine and Health Sciences, University of Gondar with approval number 435/2021. Each study participant was informed about the objective and purpose of the study which contributes necessary information for policymakers and concerned bodies. Written informed consent was obtained from each selected study participant before we asked them. Participants were informed that they have the right to continue or withdraw from the study at any time. The confidentiality of information provided by study participants was protected by making the data collection procedure anonymous and all information of the study subject would be kept confidential.
Consent for publication
Not applicable.
Competing of interest
The authors declare that they have no conflict of interest regarding the publication of this paper.
Footnotes
References
Associated Data
Data Availability Statement
The datasets used and/or analyzed during the current study are available from the corresponding author upon reasonable request.