Characterization of the Glucagonlike Peptide-1 Receptor in Male Mouse Brain Using a Novel Antibody and In Situ Hybridization

Nov 3, 2017Endocrinology

Mapping the Glucagon-like Peptide-1 Receptor in Male Mouse Brain Using New Antibody and Gene Detection Methods

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Abstract

A specific monoclonal GLP-1 receptor antibody reveals abundant expression of the receptor in several brain regions.

GLP-1 receptor (GLP-1R) expression was mapped in murine brain tissue using immunohistochemistry and nonradioactive in situ hybridization.
The GLP-1R was found in multiple brain regions, including the septal nucleus, hypothalamus, and brain stem.
Notably, GLP-1R protein was detected on neuronal projections in areas lacking detectable mRNA.
These findings enhance the understanding of GLP-1R distribution in both neuronal cell bodies and their projections.

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Glucagonlike peptide 1 (GLP-1) is a physiological regulator of appetite, and long-acting GLP-1 receptor (GLP-1R) agonists lower food intake and body weight in both human and animal studies. The effects are mediated through brain GLP-1Rs, and several brain nuclei expressing the GLP-1R may be involved. To date, the mapping of the complete location of GLP-1R protein in the brain has been challenged by lack of good antibodies and the discrepancy between mRNA and protein, especially relevant in neuronal axonal processes. Here, we present a specific monoclonal GLP-1R antibody for immunohistochemistry with murine tissue and show detailed distribution of GLP-1R expression, as well as mapping of GLP-1R mRNA by nonradioactive in situ hybridization. Semiautomated image analysis was performed to map the GLP-1R distribution to atlas plates from the Allen Institute for Brain Science. The GLP-1R was abundantly expressed in numerous regions, including the septal nucleus, hypothalamus, and brain stem. GLP-1R protein expression was also observed on neuronal projections in brain regions devoid of any mRNA that has not been observed in earlier reports. Taken together, these findings provide knowledge on GLP-1R expression in neuronal cell bodies and neuronal projections.

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Characterization of the Glucagonlike Peptide-1 Receptor in Male Mouse Brain Using a Novel Antibody and In Situ Hybridization

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