Effects of graviola oil extract on fattening performance and circadian rhythms of adipokine, cardiac and mitochondrial function markers in lambs

Mar 10, 2026BMC veterinary research

Graviola oil extract’s effects on growth, daily hormone cycles, heart, and energy markers in lambs

AI simplified

Circadian Biology on OpenScience ↗PubMed ↗DOI ↗OA ↗

Abstract

The 400 mg/kg dose of graviola oil extract significantly increased live weight in Anatolian Merino lambs by day 60.

The G400 dose showed a significant increase in live weight and live weight gain (p < 0.05).
The G200 dose improved the feed conversion ratio significantly (p < 0.05), while feed consumption remained unchanged (p > 0.05).
On day 60, the G400 dose effectively modulated the levels of BMAL1, MOTS-c, and BMCP1, with peaks occurring at 19:00.
The G600 dose produced the highest levels of Apelin (peak at 19:00) and cTnI (peaks at 13:00 and 07:00).
These findings suggest that graviola oil extract could enhance growth performance and support metabolic functions in lambs.

AI simplified

BACKGROUND: This study investigated the effects of dietary graviola (Annona muricata L.) oil extract (GOE) (supplemented at 200, 400, and 600 mg/kg) on the growth performance and circadian rhythm profiles of adipokine, cardiac, and mitochondrial function biomarkers in 48 male Anatolian Merino lambs (2.5 months old).

METHODS: Lambs were assigned to a control group (G0) or supplementation groups (G200, G400, G600 mg/kg) for a 60-day trial (70 days total). The study was conducted under a 12-h light/12-h dark cycle to ensure controlled environmental conditions. To analyze circadian profiles, blood samples were collected on days 0, 15, 30, 45, and 60 at four time points (07:00, 13:00, 19:00, and 01:00). Serum levels of Apelin, cTnI, BMAL1, MOTS-c, and BMCP1 were measured by ELISA. Data were analyzed using GLM Multivariate analysis (p < 0.05).

RESULTS: Key findings showed the G400 dose resulted in the most effective linear increase in live weight and live weight gain by day 60, and had a significant overall effect on live weight (p < 0.05). The G200 dose significantly improved the feed conversion ratio (p < 0.05), while feed consumption was unaffected (p > 0.05). Regarding biomarkers on day 60, the G400 dose effectively modulated BMAL1, MOTS-c, and BMCP1 (peak at 19:00). The G600 dose yielded the best results for Apelin (peak at 19:00) and cTnI (peaks at 13:00 and 07:00).

CONCLUSIONS: In conclusion, GOE at a dose of 400 mg/kg (G400) not only optimizes fattening performance in Anatolian Merino lambs but also supports metabolic homeostasis by modulating adipokine levels, cardiac function, and mitochondrial biogenesis processes in a manner consistent with the circadian rhythm. These findings demonstrate that the G400 dose is a safe and effective phytogenic strategy for improving animal health and production efficiency.

Full Text

Full text is available at the source.

View full text (HTML) ↗

Effects of graviola oil extract on fattening performance and circadian rhythms of adipokine, cardiac and mitochondrial function markers in lambs

Abstract

Full Text

You found one interesting study. We’ll send the next 7.

what lands in your inbox each week:

Recent issues from the circadian biology brief