Gut bacteria variety in cervical cancer patients: Comparing two DNA sequencing methods

Comparative indices showed high congruence between 16S ribosomal RNA gene sequencing and shotgun metagenome sequencing.

• The most abundant genera identified by 16S and WMS did not overlap.
• Overlap was observed at the Phylum level, which was expected.
• Relative abundances of individual genera correlated poorly between the two methodologies, with all P-values greater than 0.05.
• Hierarchical clustering revealed overlapping enterotypes from both sequencing analyses.
• Both methods agreed on demographic variables related to the patient population.

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