Diversity and composition of gut microbiome of cervical cancer patients: Do results of 16S rRNA sequencing and whole genome sequencing approaches align?

Mar 31, 2021Journal of microbiological methods

Gut bacteria variety in cervical cancer patients: Comparing two DNA sequencing methods

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Abstract

Comparative indices showed high congruence between 16S ribosomal RNA gene sequencing and shotgun metagenome sequencing.

  • The most abundant genera identified by 16S and WMS did not overlap.
  • Overlap was observed at the Phylum level, which was expected.
  • Relative abundances of individual genera correlated poorly between the two methodologies, with all P-values greater than 0.05.
  • Hierarchical clustering revealed overlapping enterotypes from both sequencing analyses.
  • Both methods agreed on demographic variables related to the patient population.

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