Hepatic overexpression of microsomal triglyceride transfer protein (MTP) results in increased in vivo secretion of VLDL triglycerides and apolipoprotein B.
Increasing liver levels of a fat-transfer protein raises blood levels of fat-carrying particles and related proteins
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Abstract
Injection of recombinant adenovirus expressing MTP (AdhMTP) resulted in a 3-fold increase in hepatic microsomal triglyceride transfer activity in mice.
- AdhMTP-injected mice exhibited significantly elevated plasma triglyceride (TG) levels compared to control mice.
- Hepatic TG secretion rates were 184 +/- 12 mg/kg/h in AdhMTP-injected mice, versus 65 +/- 9 mg/kg/h in control mice.
- Hepatic secretion of very low density lipoprotein (VLDL) apoB was 74% higher in the AdhMTP group than in the control group.
- Both apoB-48 and apoB-100 contributed equally to the increased VLDL-apoB secretion.
- These findings indicate that MTP may be a critical factor in regulating VLDL apoB secretion in normal mice.
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