Genes linked to mitochondria cleanup that may help diagnose focal segmental glomerulosclerosis

A total of 839 differentially expressed genes were identified in focal segmental glomerulosclerosis (FSGS).

• Thirty-three differential mitophagy-related genes were also identified through transcriptomic analysis.
• Machine learning identified six candidate biomarkers with moderate-to-good ability to differentiate FSGS across various groups.
• xCell analysis indicated significant renal immune changes in FSGS, including increased pro-inflammatory cells and reduced naive T cells and regulatory T cells.
• Single-cell RNA sequencing suggested a trend of decreased expression of hub genes in podocytes affected by FSGS.
• Validation through qRT-PCR and western blot confirmed downregulation of these genes in FSGS mouse models.

Simplified