We can’t show the full text here under this license.
Abstract
Lipid nanoparticles (LNPs) are recognized as a clinically validated method for delivering CRISPR cargo.
- CRISPR-based genome editing has the potential to enhance immune cell functions in treating various diseases.
- Viral vectors offer high delivery efficiency but are limited by issues such as immunogenicity and genomic integration.
- Non-viral methods like electroporation show effectiveness but are currently unsuitable for clinical use.
- Recent developments in LNPs include selective organ targeting and ligand conjugation, improving their precision in immune cell engineering.
- Challenges persist with LNPs, including difficulties in endosomal escape and extrahepatic targeting.
Simplified