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Molecular characterization of insulin-like androgenic gland hormone-binding protein gene from the oriental river prawn Macrobrachium nipponense and investigation of its transcriptional relationship with the insulin-like androgenic gland hormone gene
Molecular study of a protein that binds a male hormone and its gene activity linked to the male hormone gene in the oriental river prawn
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Abstract
Full-length cDNA of IAGBP was cloned from the oriental river prawn Macrobrachium nipponense.
- Mn-IAGBP mRNA was found in all adult male prawn tissues, with the highest levels in the testis.
- Mn-IAG mRNA was present in the androgenic gland and hepatopancreas.
- Two gene copies of Mn-IAGBP and Mn-IAG were identified through genomic analysis.
- Injection of Mn-IAGBP double-stranded RNA reduced Mn-IAG transcription significantly.
- Injection of Mn-IAG double-stranded RNA reduced Mn-IAGBP transcription significantly in several tissues.
- These findings suggest a transcriptional relationship between IAGBP and IAG in M. nipponense.
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