Intracellular calcium during fatigue of cane toad skeletal muscle in the absence of glucose

Dec 29, 2000Journal of muscle research and cell motility

Calcium levels inside muscle cells during tiredness in cane toads without glucose

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Abstract

Fatigue in single muscle fibers of cane toads resulted in a force reduction to 50% within 8-10 minutes during initial fatigue runs.

The peak levels of intracellular calcium initially increased during the first fatigue run but declined to 54±5% of the initial value when force fell to 50%.
In subsequent fatigue runs, force declined more rapidly, yet the peak tetanic calcium levels remained similar to those observed in the first fatigue runs.
After the first fatigue run, the rapidly releasable calcium store from the sarcoplasmic reticulum decreased to 46±6% of its pre-fatigue value, while this store increased to 109±16% after the final fatigue run.
The glycogen content in muscle fibers decreased from 117±17 mmol glycosyl units/l fiber after one fatigue run to 85±9 mmol glycosyl units/l fiber after the final fatigue run.
[Mg2+]i levels did not show significant changes, indicating that ATP levels may not decline significantly during either fatigue pattern.

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Mechanisms of fatigue were studied in single muscle fibres of the cane toad (Bufo marinus) in which force, intracellular calcium ([Ca2+]i), [Mg2+]i, glycogen and the rapidly releasable Ca2+ from the sarcoplasmic reticulum (SR) were measured. Fatigue was produced by repeated tetani continued until force had fallen to 50%. Two patterns of fatigue in the absence of glucose were studied. In the first fatigue run force fell to 50% in 8-10 min. Fatigue runs were then repeated until force fell to 50% in < 3 min in the final fatigue run. Addition of extracellular glucose after the final fatigue run prolonged a subsequent fatigue run. In the first fatigue run peak tetanic [Ca2+]i initially increased and then declined and at the time when force had fallen to 50% tetanic [Ca2+]i was 54+/-5% of initial value. In the final fatigue run force and peak tetanic [Ca2+]i declined more rapidly but to the same level as in first fatigue runs. At the end of the first fatigue run, the rapidly releasable SR Ca2+ store fell to 46+/-6% of the pre-fatigue value. At the end of the final fatigue run the rapidly releasable SR Ca2+ store was 109+/-16% of the pre-fatigue value. In unstimulated fibres the nonwashable glycogen content was 176+/-30 mmol glycosyl units/l fibre. After one fatigue run the glycogen content was 117+/-17 mmol glycosyl units/l fibre; at the end of the final fatigue run the glycogen content was reduced to 85+/-9 mmol glycosyl units/l fibre. [Mg2+]i did not change significantly at the end of fatigue in either the first or the final fatigue run suggesting that globally-averaged ATP does not decline substantially in either pattern of fatigue. These results suggest that different mechanisms are involved in the decline of tetanic [Ca2+]i in first compared to final fatigue runs. The SR Ca2+ store is reduced in first fatigue runs; this is not the case for the final fatigue run which is associated with a decline in glycogen and possibly related to either a non-metabolic effect of glycogen or a spatially-localised metabolic decline.

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Intracellular calcium during fatigue of cane toad skeletal muscle in the absence of glucose

Abstract

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