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Abstract
Significant differences in endosomal escape rates were observed between two DNA-loaded lipid nanoparticle formulations.
- Lipid nanoparticles (LNPs) are important for the delivery of nucleic acids in gene therapy.
- Current formulations have low endosomal escape rates, highlighting a challenge in effective delivery.
- An automated live-cell imaging method was developed to analyze the intracellular processing of LNPs.
- Fluorescent labeling allowed for tracking of DNA-loaded LNPs in engineered HuH7 hepatic cell lines.
- Subpopulations of target cells were activated for endosomal escape or recycling after treatment.
- Recycling inhibitors were utilized to enhance endosomal escape of the LNPs.
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