Magnetic targeting significantly improved kidney function in mice with cisplatin-induced (AKI).
(MSCs) labeled with showed enhanced protective effects against cisplatin-induced injury in renal tubular cells.
The application of an external magnetic field increased the homing ability of NP-labeled MSCs to kidney tissues.
Magnetically-targeted MSCs reduced oxidative stress and inflammation, leading to lower cell death and increased cell growth.
Enhanced kidney function was observed in mice treated with magnetically-targeted MSCs compared to those receiving non-targeted treatments.
Simplified
BACKGROUND: (AKI) is characterized by the abrupt loss of renal function and lack of curative therapies. Placental-derived (PL-MSCs) have shown promise in regenerative medicine, including in the treatment of AKI. However, optimizing the therapeutic effects of PL-MSCs remains a critical objective. Magnetic targeting is one potential avenue of optimization. Using iron oxide-labeled MSCs with an external magnetic field to increase cell homing ability may be an ideal method for improving the cell therapy effects in vivo.
METHODS: In this study, PL-MSCs were labeled with FeOnanoparticles coated with polydopamine (FeO@PDA NPs) for 24 h, and cell efficiency and viability were tested. The conditionally immortalized mice renal tubular endothelial cells (mRTECs) were incubated with cisplatin (Cis) and co-cultured with non-labeled or NP-labeled MSCs. The protective effect of NP-labeled MSCs on mRTEC was evaluated. In in vivo experiments, non-labeled or NP-labeled MSCs, with or without an external magnetic field, were injected into mice with Cis-induced AKI. The blood and tissue samples were collected to assess renal function and tissue damage. 3 4 3 4
RESULTS: The study confirmed that MSCs or MSC-NP can significantly improve Cis-induced mRTEC injury. In addition, NP-labeled MSCs with an external magnetic field (magnetically-targeted MSCs) improved their homing to the kidney tissues in mice with AKI, resulting in enhanced kidney function compared with those of mice treated with MSC or NP-labeled MSC treatment alone. Moreover, magnetically-targeted MSCs alleviated renal injury through suppressing oxidative stress and inflammation, reducing cell apoptosis, and promoting cell proliferation.
CONCLUSION: Magnetic targeting enhances the therapeutic effects of PL-MSCs on Cis-induced AKI in mice, suggesting that magnetically-targeted MSCs could serve as potential treatments for patients with Cis-induced AKI.
Key numbers
133.76±6.04 vs 29.07±1.48 μmol/L
Decrease in Levels
levels in mice treated with magnetically-targeted vs. untreated controls.
66.06±8.47 vs 16.39±0.58 μmol/L
Decrease in Levels
levels in mice treated with magnetically-targeted vs. untreated controls.
6.16±0.62% vs 18.33±1.24%
Reduction in Rate
rates in mice treated with magnetically-targeted vs. untreated controls.
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