Core circadian clock protein KaiC from cyanobacterium Gloeocapsa sp. PCC 7428 keeps its key functions and structure

Gl-KaiC shares basic properties with Se-KaiC, suggesting similarities in their functions.

• Gl-KaiC is a protein from Gloeocapsa sp. PCC 7428 that was successfully prepared and characterized.
• Both Gl-KaiC and Se-KaiC exhibit autokinase, autophosphatase, and ATPase activities.
• These enzymatic activities show a circadian rhythm influenced by the presence of KaiA and KaiB.
• The findings indicate that the structure and function of KaiC may vary across different cyanobacterial species.
• The established protocol can facilitate further analysis of Gl-KaiC, aiding understanding of cyanobacterial clock systems.

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