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Abstract
Lysosomal acidification in mast cells is progressively lost with age, with restoration observed following chronic ketotifen treatment.
- Mast cells from 2-month-old mice exhibited very low lysosomal acidity, indicating immature granules.
- As mast cells matured, lysosomal acidity increased, but between 15 to 17 months, a gradual deacidification was noted.
- Chronic ketotifen treatment not only restored lysosomal acidity but also enlarged granules and reduced expansion of mast cells in late life.
- Ketotifen's effects on lysosomal acidity were linked to the activity of V-ATPase and the process of endocytosis.
- Ageing in the brain was associated with hypertrophy of mast cells, yet ketotifen treatment reversed these changes toward a younger profile.
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