Krüppel-like factor 4 regulates cellular proliferation and differentiation in human bone marrow-derived mesenchymal stem cells

Sep 22, 2025Biochemistry and biophysics reports

Krüppel-like factor 4 controls growth and specialization of human bone marrow stem cells

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Regenerative Health on OpenScience ↗PubMed ↗DOI ↗

Abstract

KLF4 expression significantly decreased within 24 hours following the induction of adipogenic or osteogenic differentiation in mesenchymal stem cells.

Knockdown of KLF4 promoted cellular proliferation and differentiation at an early stage.
KLF4 may regulate both cellular proliferation and the early stages of differentiation.
The study suggests KLF4 is important for maintaining mesenchymal stem cell properties.
KLF4's regulatory role is associated with TGF-β, WNT, and FGF signaling pathways.

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Mesenchymal stem cells (MSCs) form one of the types of adult stem cell which have the capacity to self-renew, and the multipotentiality for osteogenic, chondrogenic and adipogenic differentiation and immune modulation. Consequently, MSCs are an attractive cell source for regenerative medicine and therapy for inflammatory disease. However, biological criteria to ensure any particular MSCs are "stem cells" have not been clarified. Previously, we reported that MSCs isolated from a single CD90/CD271 double-positive cell in human bone marrow have high colony-forming capacity and tri-lineage differentiation potential. Such clonal MSCs are highly homogeneous and considered to be useful for the analysis of molecular function. In this study, we focused on Krüppel-like factor 4 (KLF4) which is highly expressed by our MSC subtype, and examined its role. The expression of KLF4 was significantly reduced within 24 h after the induction of adipogenic or osteogenic differentiation. Knockdown of KLF4 led to promotion of cellular proliferation and differentiation at an early stage. Furthermore, the expressions of,,,andgenes were upregulated in KLF4 knockdown MSCs. These results indicated that KLF4 regulates not only cellular proliferation but also the early stage of differentiation. Our findings suggest that KLF4 has an important role in maintaining the properties of MSCs and regulating differentiation via TGF-β, WNT and FGF signaling pathways. in vitro in vitro TGFBR1FZD6FGFR2THY1CXCL12

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Krüppel-like factor 4 regulates cellular proliferation and differentiation in human bone marrow-derived mesenchymal stem cells

Abstract

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