Layered fluid ejection device improves production and preserves mRNA and SaRNA nanoparticle medicines

The LFE Device achieved 94% RNA encapsulation efficiency for mRNA in comparison to lower efficiencies from other delivery systems.

• High shear forces from a commercial nebulizer increased LNP particle size from 85 nm to 300 nm.
• Increased particle size and polydispersity in the nebulizer led to RNA degradation and reduced encapsulation efficiency from 100% to 39%.
• The LFE Device maintained LNP structural integrity and resulted in superior functional protein expression, with a 3-fold increase in luciferase activity compared to the commercial nebulizer.
• Selecting the appropriate delivery system is important for optimizing RNA-LNP delivery and retention in intranasal applications.
• The findings suggest the LFE Device is a promising candidate for RNA-LNP-based mucosal vaccine administration.

Simplified