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Laminar fluid ejection device enables high yield and preservation of mRNA and SaRNA LNP formulations
Layered fluid ejection device improves production and preserves mRNA and SaRNA nanoparticle medicines
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Abstract
The LFE Device achieved 94% RNA encapsulation efficiency for mRNA in comparison to lower efficiencies from other delivery systems.
- High shear forces from a commercial nebulizer increased LNP particle size from 85 nm to 300 nm.
- Increased particle size and polydispersity in the nebulizer led to RNA degradation and reduced encapsulation efficiency from 100% to 39%.
- The LFE Device maintained LNP structural integrity and resulted in superior functional protein expression, with a 3-fold increase in luciferase activity compared to the commercial nebulizer.
- Selecting the appropriate delivery system is important for optimizing RNA-LNP delivery and retention in intranasal applications.
- The findings suggest the LFE Device is a promising candidate for RNA-LNP-based mucosal vaccine administration.
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Key numbers
94%
()
Measured after dispensing with the .
~44%
RNA Recovery Rate ()
Compared to ~12% recovery with the .
60%
Functional Protein Expression ()
Measured relative to control after transfection.