Both leukocyte-poor and leukocyte-rich improved rabbit , and leukocyte-poor PRP lowered inflammatory cytokines more.
Evidence
This preclinical experiment used a papain-induced rabbit knee osteoarthritis model in 32 rabbits and compared 8-week histology, immunohistochemistry, pathology scores, and joint-fluid IL-1β and TNF-α after LP-PRP or LR-PRP injection.
Caveat
The evidence comes from an animal model, and LP-PRP did not significantly outperform LR-PRP on synovial or cartilage morphology or most other indices.
Simplified
Osteoarthritis of the knee () is a degenerative disease characterized by the deterioration, destruction, and proliferation of articular cartilage and synovium. , a crucial intracellular homeostatic mechanism, significantly contributes to the pathogenesis of OA. (PRP), derived from autologous blood, contains a high concentration of platelets that secrete various growth factors, promoting the regeneration and repair of joint structures. In this experiment, we compared leukocyte-rich platelet-rich plasma (LR-PRP) with leukocyte-poor platelet-rich plasma (LP-PRP) in a rabbit osteoarthritis model induced by papain to investigate the therapeutic efficacy and mechanism of action of platelet-rich plasma on osteoarthritis. Additionally, we analyzed whether different leukocyte concentrations in platelet-rich plasma would affect the treatment outcomes. Papain was injected into the knee joints of 32 rabbits to induce osteoarthritis, and the animals were cultured until bone maturity. LP-PRP and LR-PRP were prepared using different centrifugation methods and injected into the knee joint cavity. Eight weeks after the injection, various analyses were performed on the rabbit knee joint synovium and cartilage. These included HE staining, immunohistochemistry for PI3K, AKT, mTOR, LC3-II, MMP-13, toluidine blue staining, and ELISA for IL-1β and TNF-α in joint fluid. The synovial membrane was scored using the Krenn score, and cartilage was evaluated with the Mankin pathology score. Quantitative analysis of immunohistochemistry was conducted using ImageJ image processing software. The rabbit osteoarthritis model was successfully established by injecting papain into the knee joint cavity. All indices showed significant differences between the model group and the control group (P < 0.05). Both LP-PRP and LR-PRP exhibited therapeutic effects compared to the model group (P < 0.05). The concentrations of IL-1β and TNF-α in the LP-PRP group were significantly lower than those in the LR-PRP group (P < 0.05). However, no significant differences were observed in the morphology of the synovial membrane, cartilage, and other indices between the LR-PRP and LP-PRP groups (P > 0.05).
Key numbers
IL-1β and TNF-α levels lower in vs.
Decrease in Inflammatory Factors
Measured in joint fluid after treatment
32 rabbits
Rabbit Model Size
Total number of rabbits used in the study
Full Text
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Declarations. Ethics approval and consent to participate: The experiment was approved by the Ethics Committee of Binzhou Medical University Affiliated Hospital with approval number 20220929. Consent for publication: Not applicable. Competing interests: The authors declare no competing interests.
PubMed
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