Licochalcone A loaded multifunctional chitosan hyaluronic acid hydrogel with antibacterial and inflammatory regulating effects to promote wound healing

Nov 11, 2024International journal of biological macromolecules

Hydrogel with Licochalcone A that fights bacteria and reduces inflammation to help wounds heal

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Regenerative Health on OpenScience ↗PubMed ↗DOI ↗

Abstract

The LicA@chitosan-hyaluronic acid hydrogel significantly accelerated wound healing in mice.

The hydrogel exhibited a porous structure suitable for drug delivery.
LicA was effectively loaded into the chitosan-hyaluronic acid hydrogel.
The hydrogel demonstrated good biological safety and inhibited Escherichia coli and Staphylococcus aureus.
Positive expression of CD31, VEGF, and HIF-1α was induced, suggesting enhanced blood vessel formation.
The hydrogel down-regulated M1 macrophage markers while increasing M2 macrophage markers, indicating a shift in inflammatory response.

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The wound healing process is characterized by persistent infection and long-term inflammation. The licochalcone A (LicA) has the potential for skin wound healing and needs a good drug-loading platform to apply its antibacterial and anti-inflammatory effects. In this study, the LicA@chitosan (CS) -hyaluronic acid (HA) hydrogel with antibacterial and anti-inflammatory was developed for wound healing in mice. The SEM displayed that the hydrogel had an obvious porous structure and was very suitable to be used as a delivery carrier for LicA. The FTIR results suggested that the LicA can be effectively loaded in the CS-HA hydrogel. Variable strain scanning, frequency scanning and temperature scanning indicated that the LicA@CS-HA hydrogel can maintain the gel state. The LicA@CS-HA hydrogel had good biological safety, can inhibit the activity of Escherichia coli and Staphylococcus aureus, and can release LicA stably. The LicA@CS-HA hydrogel also has good adhesion and hemostatic properties. Finally, the LicA@CS-HA hydrogel significantly accelerated wound healing in mice skin injury model, and reduced inflammation and orderly collagen deposition were observed by HE and Masson staining. The immunohistochemistry indicated that the LicA@CS-HA hydrogel induced the positive expression of CD31, VEGF, and HIF-1α promoted neovascularization. The LicA@CS-HA hydrogel also down-regulated the expression of M1 macrophage markers CD86, IL-6, and TNF-α, and increased the expression of M2 macrophage markers CD206, IL-4, and IL-10 proteins. The molecular docking demonstrated that the target proteins had better binding activity to LicA. Collectively, the LicA@CS-HA hydrogel has broad application prospects in promoting wound healing.

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Licochalcone A loaded multifunctional chitosan hyaluronic acid hydrogel with antibacterial and inflammatory regulating effects to promote wound healing

Abstract

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