Stable mRNA vaccines without fats made using atomic layer deposition

Atomic layer deposition produced lipid-free mRNA vaccine microparticles that stayed stable at warm temperatures and triggered immune responses in preclinical testing.

Formulation and preclinical platform experiments used spray-dried polysaccharide microparticles with alumina shells carrying mRNAs for ovalbumin, N332-GT2, and GFP, with in vitro macrophage uptake, in vivo immune responses, and 6-month storage stability up to 40 °C.

The evidence is preclinical and platform-based, with model antigens and animal or cell-system endpoints rather than human vaccine efficacy.