Scientific reportsDec 22, 2025

Using fat-based particles to deliver gene-silencing RNA targeting a membrane enzyme in a human stomach cancer model in mice

mRNA Technology Weekly Brief ↗PubMed ↗DOI ↗OA ↗

Updated Jun 27, 2026

Abstract

Essence

carrying anti- siRNA improved tumor delivery and strengthened fluorouracil activity in a gastric cancer xenograft model.

Evidence

This was an in vitro and nude-mouse subcutaneous NUGC-3 gastric cancer xenograft experiment comparing LNP-loaded siRNA with naked siRNA and combination therapy with 5-FU versus 5-FU alone.

Caveat

The evidence is preclinical, uses an immunodeficient mouse xenograft model, and does not establish human efficacy or safety.

Simplified

Over-expression of (TMPRSS4), which promotes epithelial-to-mesenchymal transition and cancer cell invasion, is associated with poor prognosis in patients with gastric cancer. This study aimed to develop a new anti-TMPRSS4 small interfering RNA (siRNA) therapeutic strategy by engineering (LNPs). The biodistribution and anti-tumor effect of anti-TMPRSS4-siRNA loaded LNPs were evaluated in vitro and in vivo in mice with gastric cancer tumors (subcutaneous xenograft NUGC-3 cell tumors). LNPs demonstrated enhanced accumulation in the tumor compared with naked siRNA. A significant reduction in gastric cancer tumor growth was observed in mice undergoing combination therapy with LNPs and fluorouracil (5-FU) compared to mice receiving 5-FU alone. Anti-TMPRSS4-siRNA loaded LNPs may be considered a promising therapeutic modality for gastric cancer.

Key numbers

487.4 ± 331.7 mg

Reduction

Weight of tumors in mice treated with -siTMPRSS4 and .

962.6 ± 435.5 mg

Control

Weight of tumors in control group receiving alone.

93%

Accumulation

Encapsulation efficiency of in .

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Funding

Competing interests

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authors report competing interests

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PubMed

Using fat-based particles to deliver gene-silencing RNA targeting a membrane enzyme in a human stomach cancer model in mice

Abstract

Key numbers

Full Text

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Competing interests

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Abstract

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Funding

Competing interests

Related papers

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