α-lipoic acid reduced D-galactose-induced senescence-like injury in SH-SY5Y neuroblastoma cells.
Evidence
This in vitro cell study induced senescence with D-galactose in human SH-SY5Y cells and found 62.5 and 125 μM α-lipoic acid reduced cytotoxicity, β-galactosidase senescence, , oxidative stress markers, and Bax/p53 expression while increasing Bcl-2 expression.
Caveat
The work is limited to a D-galactose neuroblastoma cell model, so it cannot establish anti-aging or neuroprotective effects in living organisms.
Simplified
BACKGROUND AND PURPOSE: Aging is a dynamic and progressive loss of physiological integrity that leads to irreversible changes in cells and tissues, thereby increasing the risk of disability, disease, and death. Previous studies have provided evidence that D-galactose (D-gal) mimics the natural aging process in humans. On the other hand, it has been shown that α-lipoic acid (α-LA) acts as an anti-inflammatory and antioxidant compound. Therefore, this study aimed to investigate the protective effects of α-LA on D-gal-induced in SH-SY5Y neuroblastoma cells.
EXPERIMENTAL APPROACH: Senescence was induced in SH-SY5Y cells by D-gal, and the protective effects of α-LA against D-gal toxicity were evaluated by the assays of β-galactosidase, (ROS), and antioxidant parameters in SH-SY5Y cells. In addition, the mRNA expression of, andgenes was evaluated using qRT-PCR. Bax, Bcl-2p53
FINDINGS/RESULTS: The results revealed that α-LA at the concentrations of 62.5 and 125 μM reduced the cytotoxicity and senescence caused by D-gal. α-LA also effectively reduced the ROS generation compared to the D-gal group. Treatment with α-LA significantly modulated the levels of malondialdehyde, total thiol, and superoxide dismutase activity, which were altered by D-gal. In addition, treatment with α-LA decreased the expression ofandgenes, while increasing the expression of thegene. Bax p53Bcl-2
CONCLUSION AND IMPLICATIONS: Overall, the results showed that α-LA could moderate the toxic effects of D-gal by increasing the antioxidant capacity and modulating the genes involved in apoptosis, and it deserves further studies.
Key numbers
31.25 of 125 μM
Reduction in -induced cytotoxicity
α-LA concentrations tested for protective effects against .
significant reduction
Decrease in malondialdehyde () levels
levels compared between and α-LA treated groups.
significant enhancement
Increase in superoxide dismutase () activity
activity levels compared between and α-LA treated groups.
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