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An Analytical Perspective on Liquid Chromatography-Mass Spectrometry Methods for the Analysis of Bile Acids in Human Blood Samples
Analysis methods for measuring bile acids in human blood using liquid chromatography and mass spectrometry
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Abstract
Accurate quantification of total bile acids (TBA) and individual bile acid species is essential for understanding their roles in health and disease.
- Bile acids are recognized not only for aiding digestion but also as signaling molecules that can affect various metabolic processes.
- They are associated with several diseases, including gastrointestinal, metabolic, cardiovascular, and neurological disorders.
- Liquid chromatography coupled with mass spectrometry (LC-MS) provides the necessary sensitivity and selectivity to detect bile acids in human blood at trace levels.
- The systematic review evaluates LC-MS methodologies for bile acid analysis in human blood from studies published between January 2010 and April 2024.
- It emphasizes the need for standardized analytical protocols by addressing existing gaps and highlighting validation criteria.
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