Liver-focused molecular probe to visualize aging donor livers with multiple imaging methods

CyC produces a turn-on near-infrared fluorescence/photoacoustic response alongside a visible blue-to-green color change upon activation by senescence-associated β-galactosidase.

• CyC is a liver-targeted molecular probe designed for assessing hepatic senescence.
• The probe shows high sensitivity and specificity for detecting β-galactosidase activity in senescent cells.
• It provides strong signal contrast in aged mouse liver tissues.
• When applied to human liver samples, CyC effectively detects β-galactosidase activity through simple ex vivo immersion.
• This platform facilitates rapid senescence evaluation by combining molecular imaging with visual sensing.

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