Llama mRNA-LNP vaccination allows quick isolation of specific nanobodies using cell-based selection

Lipid nanoparticle (LNP)-formulated mRNA may enable potent antibody responses for challenging membrane protein targets.

• Membrane proteins are difficult to target for antibody development due to their complex structure.
• There is a need for technologies that maintain the natural shape of these proteins during the antibody discovery process.
• Nanobodies, which are single-domain fragments of antibodies found in camels, may provide advantages such as high solubility and stability.
• These nanobodies could access hard-to-reach areas on proteins, facilitating the development of effective antibodies.

Simplified